RNA Extraction From Cultured Cells

Extracting and purifying RNA from cultured cells is one of the most widely used molecular biology techniques. This has traditionally been a highly repetitive and labor-intensive procedure, requiring significant time investment, and often involving the use of toxic organic chemicals. Ambion® RNA experts have developed purification products that are optimized to provide RNA of maximum yield, purity, and integrity from cultured cells, including cell suspensions using several technology formats (organic extraction, glass-fiber columns, magnetic beads, and combinations of these). These options provide users with flexibility, throughput, and choice along both the product and price continuum.

We also offer kits for analyzing cultured cell lysates using RT-PCR, without isolating RNA. This RNA purification-free or lysate-analysis method can help you generate real-time PCR results equivalent to, or in some cases better than those from purified RNA, in significantly less time. A simple lysis step preserves RNA integrity while removing genomic DNA producing a lysate ready for the supplied reverse transcription and PCR reactions. The Cells-to-CT™ and Single Cell-to-CT™ product lines offer the cell researcher tremendous performance and handling advantages.

Get information on extraction of different RNA types, and other related technologies:

Which RNA Isolation Kit from Cultured Cells is Right for You?

  Highly pure, intact RNA Complete system—cells to qRT-PCR with SYBRÂŽ primers Complete system—cells to qRT-PCR with TaqManÂŽ Master Mix Complete system—cells to qRT-PCR from 1-10 cells
Recommendations Most Cited, Top Seller   Top Seller Top Innovation
Prep time 1 hr <10 minutes <10 minutes <10 minutes
Cell lysis method High purity organic extraction (requires alcohol precipitation) Cells-to-CTâ„ĸ lysis Cells-to-CTâ„ĸ lysis Cells-to-CTâ„ĸ lysis
Pre-amplification reagents included No No No TaqManÂŽ PreAmp Master Mix
Reverse transcription reagents included No MuLV MuLV Superscript VILOâ„ĸ
PCR Enzyme included No AmpliTaq GoldÂŽ AmpliTaq GoldÂŽ PlatinumÂŽ Taq Hi-Fi
Master mix included No SYBRÂŽ Green TaqManÂŽ TaqManÂŽ
Amount of Starting material Up to 1 x 107 cells 10–100,000 cells 10–100,000 cells 1–10 cells
Price per prep $1.56 $9.62 $13.50 $18.80
Application All RNA expression methods qRT-PCR qRT-PCR qRT-PCR
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Preserve and Stabilize Your RNA

RNAlater® Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.

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