RNA Extraction From Cultured Cells
Extracting and purifying RNA from cultured cells is one of the most widely used molecular biology techniques. This has traditionally been a highly repetitive and labor-intensive procedure, requiring significant time investment, and often involving the use of toxic organic chemicals. Ambion® RNA experts have developed purification products that are optimized to provide RNA of maximum yield, purity, and integrity from cultured cells, including cell suspensions using several technology formats (organic extraction, glass-fiber columns, magnetic beads, and combinations of these). These options provide users with flexibility, throughput, and choice along both the product and price continuum.
We also offer kits for analyzing cultured cell lysates using RT-PCR, without isolating RNA. This RNA purification-free or lysate-analysis method can help you generate real-time PCR results equivalent to, or in some cases better than those from purified RNA, in significantly less time. A simple lysis step preserves RNA integrity while removing genomic DNA producing a lysate ready for the supplied reverse transcription and PCR reactions. The Cells-to-CT™ and Single Cell-to-CT™ product lines offer the cell researcher tremendous performance and handling advantages.
Get information on extraction of different RNA types, and other related technologies:
Which RNA Isolation Kit from Cultured Cells is Right for You?
|Highly pure, intact RNA||Complete system—cells to qRT-PCR with SYBR® primers||Complete system—cells to qRT-PCR with TaqMan® Master Mix||Complete system—cells to qRT-PCR from 1-10 cells|
|Recommendations||Most Cited, Top Seller||Top Seller||Top Innovation|
|Prep time||1 hr||<10 minutes||<10 minutes||<10 minutes|
|Cell lysis method||High purity organic extraction (requires alcohol precipitation)||Cells-to-CT™ lysis||Cells-to-CT™ lysis||Cells-to-CT™ lysis|
|Pre-amplification reagents included||No||No||No||TaqMan® PreAmp Master Mix|
|Reverse transcription reagents included||No||MuLV||MuLV||Superscript VILO™|
|PCR Enzyme included||No||AmpliTaq Gold®||AmpliTaq Gold®||Platinum® Taq Hi-Fi|
|Master mix included||No||SYBR® Green||TaqMan®||TaqMan®|
|Amount of Starting material||Up to 1 x 107 cells||10–100,000 cells||10–100,000 cells||1–10 cells|
|Price per prep||$1.56||$9.62||$13.50||$18.80|
|Application||All RNA expression methods||qRT-PCR||qRT-PCR||qRT-PCR|
|Order Now||Order Now||Order Now||Order Now|
Preserve and Stabilize Your RNA
RNAlater® Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.