Plant RNA Extraction
 | Plant tissues present some unique challenges for RNA isolation compared to animal tissues and cultured cells. Their cell walls may be very difficult to disrupt, and plant cells often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect RNA quality and inhibit downstream reactions. Ambion® RNA experts have developed RNA purification products that are optimized to provide RNA of maximum yield, purity, and integrity from plants in several format options. These products help address many of the issues associated with RNA isolation from plant tissue, for high quality and high yields of plant RNA.
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Which Plant RNA Isolation Kit is Right for You?
| Great for difficult samples (conifer tissue & seeds) | Rapid & fully automated | Quick & easy to use | Efficient recovery of microRNA & small RNA | ||||
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| Top Seller |  |
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| RNA types isolated | large RNA molecules only | large RNA molecules only | large RNA molecules only | Small & large RNA molecules | |||
| Prep time | 60 minutes | <45 minutes | <20 minutes | 30 minutes | |||
| Amount of starting material | Up to 1 g | Up to 10 mg | <250 mg | 0.5 to 250 mg | |||
| Isolation method | High purity organic extraction (requires alcohol precipitation) |
Scalable, flexible format with magnetic beads | Fast, convenient silica column | Highest purity & convenience; includes organic extraction & silica column (no precipitation required) |
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| High throughput compatible | |
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| Compatible products | AmbionÂŽ Plant RNA Isolation Aid Removes common plant contaminants |
AmbionÂŽ Plant RNA Isolation Aid Removes common plant contaminants |
AmbionÂŽ Plant RNA Isolation Aid Removes common plant contaminants |
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| Price per prep | $1.36 | $3.00 | $4.50 | $6.65 | |||
| Order Now | Order Now | Order Now | Order Now |
Remove Polysaccharides
One of the major issues with plant RNA isolation is the presence of problematic biomolecules, such as polyphenolic compounds and polysaccharides. The Ambion® Plant RNA Isolation Aid minimizes many of these contaminants with the addition of polyvinylpyrrolidone, a high molecular weight polymer that binds to polyphenolics and polysaccharides and a pre-extraction spin step.
Preserve and Stabilize Your RNA
RNAlater® Solution is widely for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It minimizes the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
