pSilencer 4.1-CMV puro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (55 base pairs) between the BamH I and Hind III sites.

pSilencer 4.1-CMV puro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

Forward: 5'-AGGCGATTAAGTTGGGTA-3'

Reverse: 5'-CGGTAGGCGTGTACGGTG-3'



pSilencer 4.1-CMV puro Restriction Sites

Enzymes with single Restriction Sites

Site Enzyme
1110 Acc65I
4245 AccI
1574 AflIII
4413 AgeI
2467 AhdI
539 AloI
507 AloI`
1990 AlwNI
4464 AvrII
515 BamHI
1104 BglII
763 BsaAI
3629 BsaBI
4296 BsiWI
3542 BsmI
4439 BspMI
3897 BssHII
4217 BstEII
3788 DraIII
1118 EcoRI
462 HindIII
3528 HpaI
1114 KpnI
3537 MfeI
3981 MscI
406 NotI
1574 PciI
3508 PsiI
4436 PstI
4237 RsrII
4144 SacII
4244 SalI
1458 SapI
4436 SbfI
2947 ScaI
4518 SfiI
4433 SmaI
763 SnaBI
3271 SspI
4313 Tth111I
3636 XbaI
3066 XmnI

Non-Cutting Enzymes :

Enzyme
AarI
AfeI
AflII
AleI
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BclI
BlpI
BmgBI
Bpu10I
BsgI
BspEI
BsrGI
BstBI
BstXI
BstZ17I
Bsu36I
ClaI
EcoICRI
EcoNI
EcoO109I
EcoRV
FalI
FalI'
FseI
FspAI
MluI
NaeI
NgoMIV
NruI
PacI
PflMI
PmeI
PmlI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SanDI
SgrAI
SpeI
SrfI
SwaI
TaqII'
XcmI
XhoI
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