pSilencer™ 2.1-U6 puro Vector Map


This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 2.1-U6 puro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

T7 primer: 5'-TAATACGACTCACTATAGGG-3'

2.0rev primer: 5'-AGGCGATTAAGTTGGGTA-3'


pSilencer™ 2.1-U6 puro Restriction Sites (Vector Size: 4455 bp)

Enzymes with single Restriction Sites

Site Enzyme
3586 AccI
1250 AflIII
3416 AgeI
2143 AhdI
1666 AlwNI
3365 AvrII
461 BanHI
3387 BmtI
4204 BsaBI
3533 BsiWI
4293 BsmI
3390 BspMI
3611 BstEII
794 EcoRI
399 HindIII
4305 HpaI
4292 MfeI
3852 MscI
3383 NjeI
1250 PciI
3061 PflMI
759 PmlI
4325 PsiI
3401 PstI
3593 RsrII
3691 SacII
3585 SalI
1134 SapI
3401 SbfI
2623 ScaI
3318 SfiI
2947 SspI
3519 Tth111I
4193 XbaI
2742 XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BclI
BglII
BlpI
BmgBI
Bpu10I
BsgI
BspEI
BsrGI
BstBI
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
KpnI
MluI
NaeI
NgoMIV
NotI
NruI
PacI
PmeI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SanDI
SgfI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XcmI
XhoI
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