pSilencer™ 2.1-U6 hygro Vector Map



This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 2.1-U6 hygro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

T7 primer: 5'-TAATACGACTCACTATAGGG-3'

2.0rev primer: 5'-AGGCGATTAAGTTGGGTA-3'



pSilencer™ 2.1-U6 hygro Restriction Sites (Vector Size: 4885 bp)

Enzymes with single Restriction Sites

Site Enzyme
1250 aflIII
2143 AhdI
1666 AlwNI
3994 AsiSI
4395 AvaI
4416 AvrII
461 BamHI
239 BbeI
2648 BcgI
2682 BcgI'
4365 BclI
2204 BsaI
797 BseRI
3123 BsmI
4039 BspMI
3069 BstZ17I
399 HindIII
235 KasI
3256 NaeI
236 NarI
3254 NgoMIV
1250 PciI
4329 PshAI
3182 PsiI
4023 PstI
3945 RsrII
3578 SacII
3060 SalI
1134 SapI
4648 SexAI
237 SfoI
3994 SgfI
4397 SmaI
4421 StuI
4395 XmaI
2742 XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AgeI
AleI
AloI
AloI'
ApaI
AscI
BaeI
BaeI'
BanII
BbsI
BbvCI
BlpI
BmgBI
BmtI
Bpu10I
BsaBI
BsgI
BsiWI
BsrGI
BstBI
BstEII
BstXI
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
HpaI
KpnI
MfeI
MluI
MscI
NheI
NotI
NruI
PacI
PflMI
PmeI
PpuMI
PspOMI
PsrI
PsrI'
SacI
SanDI
SbfI
SfiI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI
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