Plant DNA Extraction
Plant DNA extraction has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions. Life Technologies offers an expansive portfolio of kits, reagents, devices for extraction, purification, analysis, and quantitation of plant DNA. Make your DNA isolation from plant samples easier on you and easier on your samples, while achieving high-yield and high-purity results with Life Technologies’ plant molecular biology reagents.
Which Plant DNA Extraction Kit is right for you?
|The most efficient lysis for large amounts of tissue||Most suitable for screening and GMO testing due to automation capabilities||Silica-based method for low- abundance plant DNA samples||Magnetic beads option for low-abundance plant DNA samples|
|Starting Material||≥100 mg||50–100 mg||100 mg||5–10 mg|
|Yield||Varies based on starting material||Up to 7 µg||Up to 14.6 µg||Up to 14.6 µg|
|Protocol Time||<60 mins||<15 mins||<40 mins||<40 mins|
|Plant Sample Types||Broad range of plant tissues (alfalfa & sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass & Arabidopsis thaliana leaves)||Some of the many species and tissues are canola, corn, cotton, sunflower, barley, rice (seeds); tomato, cucumber, lettuce, banana (leaves); rose, magnolia (flower); Acer, sycamore, grass, etc.||Chloroplasts & broad range of plant tissues (alfalfa & sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass & Arabidopsis thaliana leaves)||Chloroplasts & broad range of plant tissues (alfalfa & sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass & Arabidopsis thaliana leaves)|
|Isolation Technology||Organic extraction||ChargeSwitch® chemistry, magnetic beads||Silica spin column||Magnetic beads|
|High Throughput Compatibility||Yes||Yes||No||Yes|
|High Throughput / Automation Options||Yes||ChargeSwitch® gDNA Plant 960 Kit||96-well plate||
|Kit Sizes Available||100 mL||96 preps
|Order Now||Order Now||Order Now||Order Now|
Easy, High-Yield, High-Purity DNA Extraction From Plant Samples
Plant tissue is difficult enough to work with due to the high levels of polysaccharides and polyphenols present. Why compound the issue with tedious, inefficient processes? Cetyl trimethyl ammonium bromide (CTAB) methods require excessive time and handling, limiting your throughput. Silica membrane or magnetic bead–based protocols rely on guanidine and ethanol for DNA capture, but these reagents often don’t remove inhibitors inherent in plant samples that can carry over into the final product and interfere with downstream PCR applications. This leads to frequent sample processing failure, causing you to repeat the purification—if you have the time and sample to spare. Now, Life Technologies offers you products specifically designed for easy, high-yield, high-purity DNA purification from plant samples. Inhibitors are removed for reliable downstream results the first time. In addition, the ChargeSwitch® DNA isolation method is automation friendly so you can process hundreds of samples while obtaining the purity and yield you need. DNA extraction from plant material is recognized as a difficult task with haphazard results. Make your DNA isolation from plant samples easier on you and easier on your samples, while achieving high-yield, high-purity results with Life Technologies’ plant molecular biology reagents.
Consistently higher, cleaner DNA yields obtained with the ChargeSwitch® gDNA Plant Kit. DNA was extracted from 50 mg samples of alfalfa, spinach, and corn using the ChargeSwitch® gDNA Plant Kit or Competitor P’s magnetic silica bead kit. (A) Purified DNA (40 ng from each extraction) was loaded onto a 1% agarose gel. Yields and DNA integrity were consistent among multiple preps. Lane M: 1 Kb DNA Ladder. (B) DNA yields were measured using the Quant-iT™ PicoGreen® dsDNA Reagent.
|Other Products of Interest|
In addition to DNA and RNA isolation products, Life Technologies offers an expansive portfolio of solutions for analysis and quantitation of tissue and cultured cell genomic DNA.
For Research Use Only. Not for use in diagnostic procedures.