DNA Extraction From Cells

DNA extraction from cells is one of the most widely used molecular biology techniques. This has traditionally been a highly repetitive and labor-intensive procedure, requiring significant time investment, and often involving the use of toxic organic chemicals. Life Technologies has developed purification products that are optimized to provide DNA of maximum yield, purity, and integrity from cultured cells, including cell suspensions using several technology formats; organic extraction, silica spin columns, and magnetic beads. These options provide users with flexibility, throughput, and choice along both the product and price continuum.

Which DNA Extraction From Cells Kit is right for you?

Process the largest amount of tissue Simple, sample lysis to PCR in one well Most versatile; able to handle variety of sample types
Recommendations Most cited and cost effective Top seller
Sample Types Cells, tissue Cells, blood Cells, tissue, blood, bacteria
Protocol Time 10–30 minutes <45 minutes 15 minutes
Yield Up to 70 µg DNA
Up to 50 ng DNA/well Up to 10 µg DNA
Starting Material 1–3 x 107 cells 1–2 x 104 cells 5 x 106 cells
Isolation Technology Organic Extraction ChargeSwitch® Chemistry, coated 8-well or 96-well Silica Spin Column
High Throughput Compatible No Yes No (option available)
High Throughput/Automation NA ChargeSwitch® Direct 96 Plates PureLink® 96 Genomic DNA Kit Plates
Product Size 100 mL 96 preps (8-well)
1 plate (96-well)
10 plates (96-well)
10 preps
50 preps
250 preps
Cost/Prep $1.56 $1.18 $2.57
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PureLink® Genomic DNA Mini Kit  
Higher, more concentrated yields achieved with the PureLink® Genomic DNA Mini Kit.

Working in a GMP Laboratory?

The ChargeSwitch® EasyPlex™ is for you! ChargeSwitch® EasyPlex™ has licensing suitable for the standardized testing market. These kits are labeled with GPR (general purpose reagent), and therefore can be validated for use in standard operating procedures (SOPs) by clinical labs.

Learn more about ChargeSwitch® EasyPlex™

Resources

Other Products of Interest
In addition to DNA and RNA isolation products, Life Technologies offers an expansive portfolio of solutions for analysis and quantitation of tissue and cultured cell genomic DNA.
Technical Resources

 

For Research Use Only. Not for use in diagnostic procedures.