Quick and Easy Gel Extraction of DNA
The PureLink® Quick Gel Extraction Kit is designed to purify DNA fragments from agarose gels in less than 30 minutes. The simple procedure uses a unique silica-membrane spin column to capture and purify DNA fragments from 40 bp to 10 kb, without the need for pH adjustment. Isolated DNA is free of proteins, dye, and agarose and is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling (Figure 1).
Figure 1. Amplification of DNA isolated using the PureLink® Quick Gel Extraction Kit. PCR amplicons varying in size from 100 bp to 5.4 kb were prepared using recombinant Taq DNA Polymerase. A portion of each PCR reaction was run on a 1% UltraPure™ Agarose gel (data not shown), and amplicon bands were excised and extracted using the PureLink® Quick Gel Extraction Kit. Unpurified and gel-extracted PCR products were loaded onto a 1% agarose gel and visualized using SYBR® Safe DNA Gel Stain.
Green Benefits of the PureLink® Quick Gel Extraction Kit
- Less hazardous
- Less use of nonrenewable resources
- Less energy to produce
- Decreased fuel consumption and greenhouse gas emissions for transport
- Less waste disposal
PureLink® Combo Kit
Isolating DNA from complex PCR mixtures and recovering bands from agarose gels? Try our combo kit!
The PureLink® Quick Gel Extraction and PCR Purification Combo Kit offers the ability to perform both gel extraction and PCR purification in a single kit.
For Research Use Only. Not for use in diagnostic procedures.