PureLink® Viral RNA/DNA Mini Kit
Pure. Sensitive. Concentrated.
Choose the Kit That Best Suits Your Needs
- The PureLink® Viral RNA/DNA Mini Kit accommodates starting volumes of up to 500 µL, and elution volumes as low as 10 µL
- The PureLink® Pro 96 Viral RNA/DNA Kit can be used on a vacuum manifold or centrifuge, requires a minimum speed of just 2,250xg, and uses a bucket depth of only 50 mm, eliminating the need for high speed centrifugation with deep bucket rotors
Viral Extraction with the PureLink® Viral RNA/DNA Mini Kit
One of the most difficult viruses to extract is HBV due to the rigid protein coat. Sensitive detection requires a strong lysis and pure DNA. To look at the ability of the PureLink® Viral RNA/DNA Mini Kit to efficiently extract HBV virus, samples of infectious patient plasma were extracted side by side with plasma spiked with ZeptoMetrix's HBV nucliec acid test control (NATtrol™). HBV was detected in plasma samples diluted 1:100 from both patients (grey bars) and in NATtrol™ spiked samples (blue bars) (Figure 1).
|Figure 1. Equivalent Recovery of HBV in Patient Samples and Plasma Spiked with NATtrol™ Nucleic Acid Test Controls by ZeptoMetrix using PureLink® Viral RNA/DNA Mini Kit. HBV was extracted from infectious plasma containing 3 x 109 IU/mL and from ZeptoMetrix nucleic acid test standard (NATtrol™) for HBV containing 4 x 109 IU/mL using the PureLink® Viral RNA/DNA Kit. Recovery of HBV NATtrol™ was comparable to infectious virus in patient plasma. qPCR was performed using the Platinum® SYBR® Green qPCR SuperMix-UDG w/ROX. NATtrol™ spiked samples (grey bars), HBV infected plasma (blue bars).|
Data provided by Carleen Pope, M.S. Senior Research Scientist, ZeptoMetrix Corporation. Visit www.zeptometrix.com for more information on NATtrol™ products.