PureLink™ Viral RNA/DNA Mini Kit

PureLink Viral RNA/DNA Mini Kit

Pure. Sensitive. Concentrated.

Benefits:

  • Yields highly concentrated viral nucleic acids for greater sensitivity in detection - start with 500 µl of sample and elute in just 10 µl volume
  • One kit provides a complete solution for virus extraction - both RNA and DNA viruses can be extracted and concentrated
  • Spin column technology - achieve fast and easy purification with excellent reproducibility
  • Produces high-quality DNA and RNA - ideal for downstream applications such as real-time PCR and endpoint analysis

Equivalent Recovery of HBV in Patient Samples and Plasma Spiked with NATtrol™ Nucleic Acid Test Controls by ZeptoMetrix using PureLink™ Viral RNA/DNA Mini Kit

One of the most difficult viruses to extract is HBV due to the rigid protein coat. Sensitive detection requires a strong lysis and pure DNA. To look at the ability of the PureLink™ Viral RNA/DNA Mini Kit to efficiently extract HBV virus, samples of infectious patient plasma were extracted side by side with plasma spiked with ZeptoMetrix's HBV nucliec acid test control (NATtrol™). HBV was detected in plasma samples diluted 1:100 from both patients (grey bars) and in NATtrol™ spiked samples (blue bars) (Figure 1).

Figure 1. HBV was extrated from infectious plasma containing 3x109 IU/ml and from ZeptoMetrix nucliec acid test standard (NATtrol™) for HBV containing 4x109 IU/ml using the PureLink™ Viral RNA/DNA Kit. Recovery of HBV NATtrol™ was comparible to infectious virus in patient plasma. qPCR was performed using the Platinum® SYBR® Green qPCR SuperMix-UDG w/ROX. NATtrol™ spiked samples- greybars, HBV infected plasma- blue bars.

Data provided by Carleen Pope, M.S. Senior Research Scientist, ZeptoMetrix Corporation. Visit www.zeptometrix.com for more information on NATtrol™ products.

More Information

 

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