Influenza A (H1N1) Research

Research Tools for Influenza Detection

Influenza detection requires several distinct steps, including isolation of the viral RNA, reverse transcription of the viral RNA to cDNA, and realtime PCR-based amplification and detection of known subtype sequences. Life Technologies supplies high-performing products for each step in this process.

Products for Influenza Research Include:

Research Tools for Detection

Step 1. Isolate Viral RNA
	PureLink™ Viral RNA/DNA Mini Kit Isolates high-quality and highly concentrated viral DNA and RNA from cell-free samples such as serum, plasma, and cell culture—ideal for downstream applications, such as real-time PCR. Available in both spin-column and 96-well plate formats for fast and easy isolation
	iPrep™ PureLink™ Virus Kit The iPrep™ PureLink™ Virus Kit allows for a simple yet standardized procedure for isolating viral RNA from a range of cell-free samples using bead-based technology.
	iPrep™ Purification Instrument The iPrep™ Purification Instrument is a closed-system benchtop instrument that offers a wide portfolio of kits for fully automated nucleic acid purification. It processes up to 13 samples in less than 30 minutes, enabling sample integrity with prefilled, sealed reagent cartridges. Just load the cartridge, add samples, and select a protocol.

Step 2. Reverse Transcribe & Amplify cDNA
	SuperScript® III Platinum® One-Step qRT-PCR Kit Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format.
Step 3: Detect Influenza Subtype
Primers & Probes for H1N1 Virus Detection
	New Product - Pandemic H1N1/09 Assay Set v 2.0 This product employs assay designs licensed from the Centers for Disease Control and Prevention (CDC). Includes the primers and probes required to run the CDC Influenza 2009 A(H1N1) pdm Real-time rRT-PCR Detection Protocol. Catalog No. 4456927 - not available for sale in the US or Japan Contact your local sales representative
	New Product - Pandemic H1N1/09 Assay Controls v 2.0 Includes 5 controls (4 positive controls (1 for each assay) and 1 negative template control). Catalog No. 4456926 - not available for sale in the US and Japan. Contact your local sales representative
Kits for Influenza A Virus Screening
	Custom TaqMan® Influenza A Detection Kit 2.0 (not for sale in U.S. or Japan) For initial influenza type A screening, this kit provides a consistent, rapid, and unparalleled easy-to-use standardized procedure for detecting the presence of Influenza A viral nucleic acid targets derived from a variety of samples. These kits contain RT-PCR reagents, a positive control, and TaqMan® probes for detecting Influenza A.
Step 4: Perform Real-Time PCR
	7500 Fast DX Real-Time PCR Instrument The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, with SDS v1.4 Software, is a 96-well, 5-color real-time PCR instrument available for in vitro diagnostic use. 7500 Fast Real-Time PCR System Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
Accommodate varying sample throughputs
	7500 Fast Service Contract 7500 Fast Qualification (IQOQ) and Instrument Performace Verification Services.