E-Gel® Agarose System for Routine NA Electrophoresis

The E-Gel® electrophoresis system is ideal for routine analysis of PCR products, restriction digests, and plasmid preparations. No gels to pour, no buffer to make, no staining/destaining required, and no gel boxes to assemble—just load your sample and run on the E-Gel® electrophoresis system. The major components of the E-Gel® electrophoresis system are:

• E-Gel® iBase™ Power System
  The E-Gel® iBase™ Power System contains a base and a power supply in one device that connects directly to an electrical outlet. With an E-Gel® iBase™ Power System, separation of DNA can typically be completed in just 7 minutes, perfect for quick analysis of your DNA fragment prior to downstream applications. 

• E-Gel® Precast Agarose Gels
  Choose from E-Gel® EX, E-Gel® with SYBR® Safe, and E-Gel® single comb and double comb gels. Learn more about our complete offering of E-Gel® Precast Agarose Gels. If you’re looking for speed and sensitivity, our E-Gel® EX gels are for you, with separation typically completed in 10 minutes.

• Separation of DNA or RNA samples typically in 10 minutes
• Avoid the hazards of UV exposure and get greater sensitivity
• Access gels easily and safely for use in downstream applications

E-Gel® EX gels offer complete resolution of DNA or RNA samples typically in just 10 minutes. These gels are ideal for a quick check of the integrity of RNA samples or routine analysis of PCR products and plasmid preparations (Figure 1). E-Gel® EX gels were developed for superior sensitivity over comparable gels containing ethidium bromide (Figure 2), allowing you to use less of your valuable sample.

		Figure 1. Check the integrity of your RNA samples using E-Gel® EX gels. RNA samples were separated on a 2% E-Gel® EX gel using the preset E-Gel® EX program on the E-Gel® iBase™ Power System. Lanes M, 5, and 10: 0.1–2 kb RNA Ladder; lanes 1–4, 6–9: mouse total RNA, 200 ng per lane.
		Figure 2. Sensitivity of DNA detection with E-Gel® EX Gels. Two-fold serial dilutions of a 1 kb fragment, ranging from 1,000 ng to 1 ng, were separated on a 1% E-Gel® EX Gel. Blue-light transillumination (with a Safe Imager™ Blue-Light Transilluminator) was used during documentation.

Results in a fraction of the time with the E-Gel® iBase™ Power System
Separate 50 bp to 10 kb samples typically in just 7 minutes, using the “Speed E-Gel®” preset program on the E-Gel® iBase™ Power System (Figure 3). Choose 0.8%, 1.2%, or 2% agarose concentrations, and E-Gel® gels incorporated with either SYBR® Safe stain or ethidium bromide.

Figure 3. Separation was carried out on a 2% E-Gel® agarose gel using the "Speed E-Gel®" preset program on the E-Gel® iBase™ Power System. Left lane: Low DNA Mass Ladder (Cat. No.10068-013) containing DNA bands of 2 kb, 1.2 kb, 800 bp, 400 bp, 200 bp, and 100 bp. Right lane: 904 bp PCR product.