Below are some frequently asked questions and answers regarding the E-Gel® Imager System and Software. If you have a question that is not listed below or need additional information, please contact our Technical Support department.
E-Gel® Imager System FAQs
- What components come with my system?
- Can I get other bases separately?
- Can the UV light base be used for both ethidium bromide–stained and SYBR® dye–stained gels?
- Are there other filters available?
- Does this system only work with E-Gel® precast agarose gels?
- Can the E-Gel® Imager System be used with proteins?
- Does it have it to be Qdot® 625 conjugate What is the emission spectra range? Which light base do you recommend? ?
- Is the E-Gel® Imager compatible with protein ECL or AP detection systems?
- How large is the E-Gel® Imager System?
E-Gel® Imager Software FAQs
- What are the two software programs that are used with the E-Gel® Imager System?
- What are the computer requirements to run the GelQuant™ Express Analysis Software?
- Can I run the software on a Macintosh® computer?
- How do I install GelCapture™ software on the Windows ® 7 Professional operating system?
- What is the dongle used for?
- What adjustments can be made to the image using the GelCapture™ software?
- What are the two main analysis options for the GelQuant™ Express Analysis Software?
- Does the GelQuant™ software in the E-Gel® Imager System work for protein analysis such as quantification?
- Where can I get more information about these software programs?
There are three full-system options to choose from:
Yes, when using both ethidium bromide–stained and SYBR® dye–stained gels, we recommended using the UV Light Base with the Universal (orange) Filter for the ethidium bromide–stained gels and the green filter for the SYBR® dye–stained gels. Use of the blue-light base for ethidium bromide–stained gels is not recommended, as the fluorescence signal emitted from the ethidium bromide–stained bands using a blue-light base is a tenth of the signal obtained with the UV light base.
Yes, there are three filters available for use with this system. In addition to the Universal filter, the E-Gel® Imager Qdot® 625 filter and the E-Gel® UV/SYBR® Filter are available. All three filters can be purchased separately.
No. This system is not only appropriate for imaging nucleic acid separation in E-Gel® precast agarose gels, it’s also a perfect solution for documenting and analyzing nucleic acids in other agarose gels, such as NuPAGE® Bis-Tris Gels and pour-your-own gels (agarose or polyacrylamide).
Yes, Using E-Gel® Imager white light conversion screen, cat # 4473061, this accessory can convert the blue light emitted by the E-Gel® Imager blue-light base, as well as the UV light emitted by the E-Gel® Imager UV-light base, to white light. The E-Gel® Imager white light conversion screen is compatible with protein gel stains such SimplyBlueTM Safe, SilverQuestTM Silver, SYPRO® Ruby and Coomassie blue stains. Additionally, any dye in the 625 emission range will work, including WesternDot™ 625 Western Blot Kit.
The E-Gel® Imager System currently only works with proteins coupled to Qdot® nanocrystals. Using the SpectraViewer tool on the Life Technologies website (lifetechnologies.com/spectraviewer), we can see that the Ex/Em peaks for Qdot® 625 conjugate are <300 nm and ~621 nm, respectively. Either the UV or the blue-light base can be used with the Qdot® nanocrystals, but the UV base will provide slightly better signal intensity.
The E-Gel® Imager will not work with the ECL or AP detection systems.
How large is the E-Gel® Imager System?
The E-Gel® Imager System is a compact benchtop device that stands 43.7 cm in height (overall dimensions: 43.7 x 28.4 x 20.3 cm). It consists of the E-Gel® Imager camera hood (35.6 cm in height) that fits on top of a base unit (11.9 cm in height).
GelCapture™ Imaging Software is used for the visualization and documentation of nucleic acid gels.
GelQuant™ Express Analysis Software is used for quantification analysis of nucleic acid gels.
Your computer will need to be running the Windows® XP professional or Windows® 7 professional 32-bit operating system in order to install and run the GelQuant™ Express software for gel capture and band quantification. Additionally, you will need to have administrative privileges on the computer.
GelQuant™ Express software requires the Windows® XP professional or Windows® 7 professional 32-bit operating system. It can run on Macintosh® computers, but only through one of these operating systems.
Installing GelCapture™ software from the CD (Windows ® 7 Professional)
- Launch the CD with the Internet Explorer® browser, right-click the GelCapture.exe installation file, and select the “Run as administrator” option.
- Select “Allow, I trust this program. I know where it’s from or I’ve used it before.” to allow the program to run.
- Follow the instructions provided by the installation wizard.
- Once installation is complete, click “Finish” to exit Setup.
The E-Gel® Imager Quantitation Dongle, also known as the GelQuant™ Express software activation dongle, must be inserted into the computer in order to use GelQuant™ Express software. If the software dongle is not detected, the warning “Sentinel HASP key not found (H007)” will appear on screen.
Exposure, sensitivity, and brightness can be adjusted using the GelCapture™ software. Captured images can then be exported to the GelQuant™ Express software for data analysis.
The 1D option is used for analyzing 1D gels, such as molecular weight determination. The Express option is designed for analysis of nucleic acids and protein gel images. In the Express mode, lane profiles are analyzed with the flexible adjustment of background values. Separate bands can be quantified as one entity for easier analysis of complex results (e.g., degraded or smear bands).
The GelQuant™ software can be used for quantifying protein gels. The image needs to be available in .jpeg or .tiff format. However, the E-Gel® Imager is not recommended for the acquisition of protein gel images, which typically require white-light illumination.
From the manuals and quick reference card. Download here:
For Research Use Only. Not for human or animal therapeutic or diagnostic use.