SYBR® Safe DNA gel stain—Better for the Environment
 | Environmental impact results (USA)Based on extensive environmental safety testing, SYBR® Safe DNA gel stain is not classified as hazardous waste under U.S. Federal regulations (Resource Conservation and Recovery Act (RCRA)). SYBR® Safe DNA gel stain meets the requirements of the Clean Water Act and the National Pollutant Discharge Elimination System (NPDES) requirements.
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Conclusions
- SYBR® Safe DNA gel stain is not classified as corrosive, ignitable, or reactive under the guidelines of the Environmental Protection Agency (EPA) (Table 1).
- SYBR® Safe DNA gel stain does not differ significantly from 0.5X TBE buffer when tested according to National Pollutant Discharge Elimination System (NPDES) guidelines (Table 2).
- SYBR® Safe DNA gel stain in 0.5X TBE is indistinguishable from 0.5X TBE alone in terms of organic pollutant content both samples tested negative for the presence of an extensive array of organic compounds (Table 3).
Table 1 - SYBR® Safe DNA gel stain hazardous waste analysis
| Analysis* | Method | Results |
|---|---|---|
| Corrosivity | EPA 150.1 | Not corrosive (pH = 8.25) |
| Corrosivity (by Corrositex) | DOT-E 10904 | Category 2 noncorrosive |
| Ignitability | EPA 1010 | Not ignitable (<212°F) |
| Reactivity | EPA 9010B/9030A | No reactivity detected |
* All tests were performed by AMEC Earth and Environmental San Diego Bioassay Laboratory, San Diego, CA.
Table 2 - SYBR® Safe DNA gel stain—Overview of NPDES analysis*
| Test | SYBR® Safe Stain in 0.5X TBE | 0.5X TBE |
|---|---|---|
| PH (150.1) | 8.45 | 8.48 |
| Total cynaide (335.2) | None detected | None detected |
| Chemical oxygen demand (COD; 410.1) | 7020 | 6840 |
| Ammonia as nitrogen (350.1) | 253 | 248 |
| Total organic carbon (415.1) | 2480 | 2360 |
| Total phenoloics (420.1) | None detected | None detected |
| Organochlorine pesticides and PCBs (608M) | None detected | None detected |
| Semi-volatile organic compounds (625) | None detected | None detected |
| Volatile organic compounds (624) | None detected | None detected |
| Priority Pollutant Metals (Sb, As, Be, Cd, Cr, Cu, Pb, Hg, Ni, Se, Ag, Tl, Zn) (EPA 200.7/200 series) | None detected | None detected |
* All tests were performed by Columbia Analytical Services, Inc., Kelso, WA. Methods used were as outlined in the Code of Federal Regulations (CFR) Title 40, Part 136.
Table 3 - SYBR® Safe DNA gel stain—Detailed organic compound analysis*
| Organochlorine pesticides and PCBs (608M) | ||||
| alpha-BHC | beta-BHC | gamma-BHC | delta-BHC (Lindane) | Heptachlor |
| Aldrin | Heptachlor Epoxide | Endosulfan I | Dieldrin | 4,4'-DDE |
| Endrin | Endosulfan II | 4,4'-DDD | Endrin Aldehyde | Endosulfan Sulfate |
| 4,4'-DDT | Toxaphene | Chlordane | Aroclor 1016 | Aroclor 1221 |
| Aroclor 1232 | Aroclor 1242 | Aroclor 1248 | Aroclor 1254 | Aroclor 1260 |
| Ethylbenzene | Bromoform | 1,1,2,2,-Tetrachloro ethane | 1,3-Dichloro benzene | |
| Volatile organic compounds (624) | ||||
| Chloromethane | Vinyl Chloride | Bromomethane | Chloroethane | Trichlorofluoro methane |
| 1,1-Dichloroethene | Methylene Chloride | trans-1,2-Dichloroethene | 1,1-Dichloroethane | Chloroform |
| 1,1,1-Trichloroethane (TCA) | Carbon Tetrachloride | Benzene | 1,2-Dichloroethane (EDC) | Trichloroethene (TCE) |
| 1,2-Dichloropropane | Bromodichloro methane | 2-Chloroethyl Vinyl Ether | trans-1,3-Dichloro- propene | Toluene |
| cis-1,3-Dichloro propene | 1,1,2-Trichloroethane | Tetrachloroethene (PCE) | Dibromochloro methane | Chlorobenzene |
| 1,4-Dichlorobenzene | 1,2-Dichlorobenzene | Acrolein | Acrylonitrile | |
| Semi-volatile organic compounds (625) | ||||
| N-Nitrosodimethyl- amine | Bis(2-chloroethyl) Ether | Phenol | 2-Chlorophenol | Bis (2-chloroisopropyl) Ether |
| Hexachloroethane | N-Nitrosodi-n-propyl- amine | Nitrobenzene | Isophorone | 2-Nitrophenol |
| 2,4-Dimethylphenol | 4-Nitrophenol | 2,4-Dichlorophenol | 1,2,4-Trichlorobenzene | Naphthalene |
| Hexachlorobutadiene | 4-Chloro-3-methylphenol | Hexachlorocyclo- pentadiene | 2,4,6-Trichlorophenol | 2-Chloronap hthalene |
| Acenaphthylene | Dimethyl Phthalate | 2,6-Dinitrotoluene | Acenaphthene | 2,4-Dinitrophenol |
| Bis (2-chloroethoxy) methane | 2,4-Dinitrotoluene | Fluorene | Diethyl Phthalate | 2-Methyl-4,6-dinitrophenol |
| N-Nitrosodiphenyl amine | 4-Bromophenyl Phenyl Ether | Hexachlorobenzene | Pentachlorophenol | Phenanthrene |
| Anthracene | Di-n-butyl Phthalate | Fluoranthene | Benzidine | Pyrene |
| Butyl Benzyl Phthalate | 3,3'-Dichlorobenzidine | Benz(a)anthracene | Chrysene | Bis(2-ethylhexyl) Phthalate |
| Di-n-octyl Phthalate | Benzo(b)fluoranthene | Benzo(k)fluoranthene | Benzo(a)pyrene | Indeno(1,2,3-cd)pyrene |
| Dibenz(a,h)anthracene | Benzo(g,h,I)perylene | 1,2-Diphenylhydrazine | 2,3,7,8-Tetrachloro- dibenzo-p-dioxin | 4-Chlorophenyl Phenyl Ether |
* Samples analyzed were 0.5X TBE and 0.5X TBE + 1X SYBR® Safe DNA gel stain; none of the compounds listed in the table were detected in either sample. Testing was performed by Columbia Analytical Services, Inc., Kelso, WA. Methods used were as outlined in the Code of Federal Regulations (CFR) Title 40, Part 136.
