Plasmid DNA Purification
Plasmid DNA Purification is a technique used to prepare DNA for downstream applications such as PCR, cloning or transfection experiments. Invitrogens range of Plasmid DNA purification kits are designed to isolate plasmid DNA and provide DNA purity you require in the scale s and format you need.
| Format | Advantages | Protocol Time | Yield | Sample Volume | Kit |
|---|---|---|---|---|---|
| Molecular biology grade (e.g. cloning, sequencing, PCR…) plasmid minipreps | |||||
| Spin/Vacuum-Column | Quick, easy, cost-effective protocols. | <25 min | up to 40 µg | 1-5 ml | PureLink™ Quick Plasmid Miniprep Kit |
| Transfection grade plasmid minipreps | |||||
| Spin-Column | High quality plasmid DNA using ChargeSwitch® in a column format. | <30 min | up to 20 µg | 1-5 ml | ChargeSwitch® -Pro Plasmid Miniprep |
| Spin-Column | Very high yields. High purity | <30 min | up to 60 µg | 1-3 ml | PureLink™ HQ Plasmid Miniprep Kit |
| Gravity-Flow Column2 | Ultrapure™ plasmid DNA | <2 hr | up to 30 µg | 1-3 ml | PureLink™ HiPure Plasmid Miniprep Kit |
| Magnetic Beads1, 3 | Low endotoxin. Scalable. ChargeSwitch® Technology | <20 min | up to 20 µg | 1-5 ml | ChargeSwitch® Plasmid ER Mini Kit |
| High-throughput plasmid minipreps | |||||
| Spin/Vacuum-Column | Spin/Vacuum-Column Rapid purification of plasmid DNA. Quick and simple lysate clarification filter | 90 min | up to 15 µg | 1.2-1.5 ml | PureLink™ Quick96 Plasmid Kit |
| 96-well Plate (vacuum/centrifuge) | Higher yields and purity. Transfection-grade plasmid DNA. | <30 min | up to 10 µg | 1-3 ml | PureLink™ HQ 96 Plasmid Miniprep Kit |
| Magnetic Beads1, 3 | Centrifuge-free protocol. Molecular biology-grade plasmid DNA. | 15 min | up to 5 µg | 0.5-1.5 ml | ChargeSwitch® No Spin Plasmid Micro Kit |
| Magnetic Beads1, 3 | Transfection-grade plasmid DNA. | <20 min | up to 20 µg | 1-5 ml | ChargeSwitch® Plasmid ER Mini Kit |
| 384-well Plate (centrifuge) | Simple protocol. Molecular biology-grade plasmid DNA. | <60 min | up to 3 µg | 200 ul | PureLink™ 384 Plasmid Purification Plates |
| Yeast plasmid minipreps | |||||
| Magnetic Beads1, 3 | Isolation of plasmid from Yeast | 20 min | up to 25 µg | 1 ml | ChargeSwitch® Plasmid Yeast Mini Kit |
| Other small-scale plasmid DNA preparation | |||||
| Reagent (liquid) | Rapid method for screening recombinant bacterial colonies for the presence of target plasmid DNA | < 1 hr | up to 20 ng for DNA analysis | 1 bacterial colony | CloneChecker™ System |
| Format | Advantages | Protocol Time | Yield | Sample Volume | Kit |
|---|---|---|---|---|---|
| Transfection grade plasmid midipreps | |||||
| Gravity-flow column format2 | Ultrapure™ Plasmid DNA | n/a | 25-100 ml | up to 200 µg | PureLink™ HiPure Plasmid Midiprep |
| Gravity-flow column format2 | Rapid purification of Ultrapure™ plasmid DNA. Simple, integrated lysate clarification filter | 60 min | 25-100 ml | up to 200 µg | PureLink™ HiPure Filter Plasmid Midiprep |
| Format | Advantages | Protocol Time | Yield | Sample Volume | Kit |
|---|---|---|---|---|---|
| Transfection grade plasmid maxi, mega and gigapreps | |||||
| Gravity-flow column format2 | Ultrapure™ Plasmid DNA | Protocol time: 60 min | up to 750 µg | 100 - 500 ml | PureLink™ HiPure Plasmid Maxiprep Kit |
| Gravity-flow column format2 | Rapid purification of ultrapure plasmid DNA. Bacterial lysate clearing without centrifugation. | 60 min | up to 850 µg | 100 - 500 ml | PureLink™ HiPure Filter Plasmid Maxiprep Kit |
| Gravity-flow column format2 | Fastest purification of Ultrapure™ plasmid DNA. Bacterial lysate clearing and isopropanol precipitation without centrifugation. | 60 min | up to 850 µg | 100 - 500 ml | PureLink™ HiPure FP (Filter and Precipitator) Maxiprep Kit |
| Vacuum Filter | Rapid purification of Ultrapure™ plasmid DNA. Simple lysate clarification filter | 2 hr | up to 2.5 mg | =2.5 liter | PureLink™ HiPure Plasmid Megaprep Kit |
| Vacuum Filter | Rapid purification of Ultrapure™ plasmid DNA. Simple lysate clarification filter | 2 hr | up to 10 mg | =5 liter | PureLink™ HiPure Plasmid Gigaprep Kit |
| 1 MagnaRack™ Magnetic Rack | For use with ChargeSwitch® products | MagnaRack™ Magnetic Rack |
| 2 PureLink™ Nucleic Acid Purification Rack | For use with PureLink HiPure Mini, Midi and Maxiprep columns | PureLink™ Nucleic Acid Purification Rack |
| 3 96-well magnetic separator | For use for automation of most ChargeSwitch® products | 96-well magnetic separator |
| PureLink™ HiPure Precipitators | For precipitation and desalting of plasmid DNA. | PureLink™ HiPure Precipitator Module |
| PureLink™ HiPure Buffer Kit | Additional PureLink™ HiPure buffers for larger culture volumes. | PureLink™ HiPure BAC Buffer Kits |
