ChargeSwitch® EasyPlex
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ChargeSwitch® EasyPlex™ gDNA Kits
- ChargeSwitch® EasyPlex™ gDNA Kits offer faster turnaround time—go from primary sample to PCR set-up five times faster.
- Increased reliability—no risk of plate blockages, bead carryover, or ethanol contamination
- Flexible automation options—easy robotic set-up; no special equipment required; increased throughput compared to silica plates
Spend less time setting up and more time analyzing results
No beads, no membranes, no centrifugation, no vacuum manifold—not even a transfer between tubes or plates. Get your results faster than ever. With the ChargeSwitch® EasyPlex™ gDNA Kit’s simple protocol, you’ll save time by going directly from genomic DNA (gDNA) isolation to PCR in one well, in less than 45 minutes.
Thank you for your interest in the ChargeSwitch® EasyPlex™ gDNA Kit. FREE samples are available for those who qualify. To receive your sample, please fill out our brief questionnaire, and a Technical Specialist will contact you to discuss your request.
ChargeSwitch® EasyPlex™ ABI Quantifiler Analysis
| Figure 4. Preparing gDNA from 10µl human blood. |
ChargeSwitch® EasyPlex™ Automation Flexibility
The ChargeSwitch® EasyPlex™ gDNA Kit is easy to automate on most automated liquid handling work stations, without the need for any special equipment for processing (such as a vacuum manifold, shaker, or centrifuge). Depending on your deck and pipetting configuration, a single plate can take as little as 30 minutes to process, after sample lysis.
| Optimized ChargeSwitch® EasyPlex™ Kit protocol for use with the Tecan Freedom Evo® instrument. |
ChargeSwitch® EasyPlex™ Viral RNA/DNA Kit
The newest revolution in viral nucleic acids research, specifically designed for small samples (≤ 30 µl)
- Direct amplification from viral nucleic acids bound to a microtiter plate surface without any elution— obtain pure, undiluted viral nucleic acids
- Fast processing with minimal handling—from lysis to PCR within an hour
- Flexible automation—easy set-up on most automated liquid handling workstations without the need for vacuum manifolds, shakers, and centrifugation
With the ChargeSwtich® EasyPlex™ system, the entire sample is bound to the plate surface, enhancing sensitivity by concentrating minute amounts of nucleic acid. The ChargeSwitch® EasyPlex™ Viral Kit demonstrates linear and sensitive amplification down to just 100 copies of Lentivirus in serum and plasma (Figure 1).
| Figure 1. Sensitive and linear viral quantitation with 10ul of serum or plasma using the ChargeSwitch® EasyPlex™ Viral RNA/DNA Kit. Lentivirus (RNA virus) was inoculated into plasma and serum and 10 µl sample was used for processing. Direct amplification of RNA in the well was performed in 50ul volume using the SuperScript™ III Platinum One-Step Quantitative RT-PCR System for qRT-PCR. |
How ChargeSwitch EasyPlex Works
| The ChargeSwitch® Technology features a charged nucleic acid–binding surface that is “switchable” by changing the pH of the surrounding buffer. At low pH, the surface is positively charged and binds the negatively charged nucleic acid backbone, allowing easy removal of proteins and other contaminants using a simple wash step. The microplates included in the ChargeSwitch® EasyPlex™ gDNA Kit are coated with this unique, acid-binding, ChargeSwitch® surface. |
ChargeSwitch® EasyPlex™ Workflow
High-performance gDNA without elution, contamination, and false positives
ChargeSwitch® kits come with aqueous buffers that do not contain guanidine or ethanol, which can inhibit downstream applications. Genomic DNA can be isolated from as little as 10µl whole blood or 1-2 x 104 cells per well. Purified gDNA is amplified directly from microplate wells or 8-well strips, without elution, and is suitable for downstream procedures such as multiplex PCR, qPCR, STR (short tandem repeat) analysis, WGA (whole genome amplification), and sequencing. In addition, ChargeSwitch® EasyPlex™ gDNA Kits are compatible with most automated liquid handling workstations, and contain semi-skirted microplates and 8-well strips that fit into most thermal- and real-time- cyclers for high-throughput procedures. With the ChargeSwitch® EasyPlex™ gDNA Kit, you get successful, highly-pure gDNA results using less sample, contamination, sample error, and false positives.
| Figure 2. From sample lysis to PCR in just one well. Using the ChargeSwitch® EasyPlex gDNA Kit, you can lyse and purify gDNA from up to 96 samples in <45 minutes. Add PCR components directly to the wells of the same plate. The elimination of transfer steps means a reduced risk of contamination and sample mix-ups. |
ChargeSwitch® EasyPlex™ Multiplex PCR with Cystic Fibrosis Assay
| Multiplex PCR performed directly on genomic DNA. For more information or to order, visit ChargeSwitch® EasyPlex™ gDNA Kit |
