GeneArt® Mutagenesis Service
 | Site-Directed MutagenesisThe GeneArt® site-directed mutagenesis service introduces single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequences quickly and economically. |
We use a targeted PCR-based process to introduce genetic variation precisely where you need it, then clone the construct into the vector of your choice. All constructs are then 100% sequence-verified and documented to help assure that the DNA you receive is exactly what you’ve requested.
GeneArt® Services provides a full range of cloning, optimization, and protein expression options to serve many areas of biological research and production.
Two Ways to Get Your Project Started
| When setting up your project online, add “single variant” or “variant bundle” to your project and follow the instructions to enter your sequence(s) |
| Download the Multiple Sequence Submission Form, enter your DNA sequence request(s) using Microsoft Excel, and email it to our customer support team. |
For further information regarding this service or the order process, please contact christian.kranz@lifetech.com.
What You Receive
 | GeneArt® Mutagenesis Service |
 | GeneArt® Mutagenesis 6+ Service —Discounted subcloning into your vector for 6 or more related constructs —5 µg DNA of each construct —100% sequence verified |
Applications
- Investigate active sites, structure-function relationships, nucleic acid–protein interactions, etc.
- Test functional elements (promoter regions, terminator sequences, etc.)
- Construct fusion proteins and tagged proteins
- Design knockout constructs
- Generate alternative splice forms
- Conduct alanine scans to identify protein functional domains
Advantages
- Economical alternative to synthetic gene construction for constructs smaller than ~1 kb
- Mutations exactly where you need them, without the risk of introducing unwanted mutations
- Unlike “quick change” strategies, the vector is not subjected to PCR—just the insert
- Insert size can be as large as 6–8 kb
- No mutations in the vector backbone
- Mutations can be clustered or far apart, with the same cost and production time
- Fast turnaround times
- Template constructs are stored for at least 2 years at no charge and longer-term storage is available
- Full sequence verification of insert DNA
- Competitive pricing
