GeneArt® Combinatorial Libraries

Maximum Diversity

Degenerated libraries constructed using GeneArt® Combinatorial technology are synthesized de novo and therefore based on true rational design. By leaving the coding framework untouched and accurate, it is possible to rationally degenerate only the portions of the gene that are likely or known to contribute to a protein's function.
Combinatorial libraries are constructed with a high degree of mutagenesis at very targeted locations to introduce maximum variation in the portions of the gene that you specify. They are available cloned into the vector of your choice or ready to clone in your laboratory. As with other GeneArt® services, constructs are subjected to rigorous quality control checks that include sequencing and statistical analysis to help assure that you receive degenerated sequence libraries that help you achieve your research goals.

Libraries Are Available Cloned or Ready to Clone

GeneArt® Ready-to-Clone Combinatorial Library
  • 5 µg of linear DNA ready to clone via 5' and 3' restriction sites
GeneArt® Cloned Combinatorial Library
  • 10 µg of plasmid DNA cloned into the vector of your choice
  • Includes glycerol stock of all library transformants

Get Your Project Started

          

Please download the questionnaire to submit project information. For secure data transfer please register at our customer portal.

For further information regarding this service or the order process, please contact christian.kranz@lifetech.com.

 

Applications

  • Diversify antigen-binding sites of antibodies
  • Improve physicochemical protein properties such as solubility, heat stability, and activity in industrial environments
  • Enhance function, such as promoter activity and enzymatic properties
  • Optimize antibody affinity (in vitro affinity maturation)
  • Humanize antibodies
  • Prolong half-life of therapeutics for in vivo environments
  • Improve enzyme function for industrial use
  • Modify enantioselectivity of enzymes
  • Induce changes to alter proteins from patented sequences

Advantages

  • Library diversities of up to 1011
  • Site-specific randomization
  • Low ancillary mutation rate
  • Fast production times

Quality Control

All GeneArt® Combinatorial Library products are sequenced and subjected to statistical analysis to help ensure that they meet the following quality benchmarks:

  • Sequencing of up to 96 individual transformants to verify that customer’s specifications regarding nucleotide content and amino acid encoding have been met, and that sequence integrity of unmutated portions of the construct meets minimum requirements
  • Bulk sequencing to verify that nondegenerated portions of the construct have the correct sequence and randomized portions meet customer’s specifications and maintain an open reading frame
  • Real-time PCR prior to amplification to verify that library diversity objectives have been met