GeneArt® Genes to Proteins
 | Fast, Reliable Protein Production From Mammalian Cell LinesThe GeneArt® Genes-to-Proteins Service is an extremely fast way to obtain protein from transient expression in mammalian cell lines. We clone your sequence-optimized gene into one of our in-house vectors, then employ advanced transfection and culture conditions to obtain high expression yields. Secreted protein from the culture supernatant is then purified using affinity or ion-exchange chromatography. |
On request, we can use your expression vector for the Genes-to-Proteins Service rather than our in-house vector. For this type of project, both the protein of interest and custom-vector expression constructs are supplied.
How To Get Your Project Started
| Download and fill out the Protein Production Request Form, then email it to the GeneArt® Cell Lines and Protein Services team geneartprotein@lifetech.com. We will respond with a price and production time quote. |
| Service | Description | Deliverables | |
|---|---|---|---|
Genes-to-Proteins Pilot | Feasibility study for determination of production yield of protein from transiently transfected HEK293 or CHO cells | Price quote for production of a minimum protein amount | |
| Genes-to-Proteins Purification | Protein purified from transiently transfected HEK293 or CHO cells, using the culture volume you specify Protein purification via affinity tag, Protein G/A, or ion-exchange chromatography Quality assurance by SDS-PAGE or analytical gel filtration | All protein obtained Cell pellet or culture supernatant can also be provided Yield and quality report | |
| Genes-to-Proteins Complete | Protein expression and purification from transiently transfected HEK293 or CHO cells Genes-to-Proteins Pilot Service required Protein purification via affinity tag, Protein G/A, or ion-exchange chromatography Quality assurance by SDS-PAGE or analytical gel filtration | Purified protein: as determined using the Genes-to-Proteins Pilot Service Comprehensive report |
For more information on the GeneArt® Genes-to-Proteins Service or to receive information on pricing and production time, please email geneartprotein@lifetech.com.
