GeneArt® Genes-to-Expression Service
Improved Protein Expression Through Gene Optimization
The GeneArt® Genes-to-Expression Service is designed to help you find the best mammalian-cell expression plasmid for your protein.
The Best-Expresser Service compares the performance of genes optimized using different strategies (Figure 1). Our standard optimization service reliably improves protein expression from most genes; however, comparison of the different optimization strategies may be beneficial in order to identify the best approach for a specific gene.
Tell us about your project for more information and to receive information on pricing and production time.
|Expression Verification Service||Transient transfection of expression construct in HEK293 or CHO cells|
Analysis of expressed protein by SDS-PAGE or
western blot, using antibodies targeting either the protein tag or the protein itself
|Best-Expresser Service||Expression comparison of different versions of your gene optimized with different strategies using the GeneOptimizer® process; all versions cloned into the same vector|
Transient transfection of expression constructs in HEK293 or CHO cells, including a time-course analysis
Relative quantification of protein expression by western blot analysis using specific anti-tag or anti-protein antibody
|Report with Best Expresser identified|
|Best-in-Class Service||Expression comparison of several protein variants (for example, expressed from truncated or DNA-shuffled constructs) cloned into the same expression vector|
Transient transfection of expression constructs in HEK293 or CHO cells, time course to determine maximal expression
Relative quantification of protein expression by western blot analysis using specific anti-tag or anti-protein specific antibody
|Report with Best in Class identified|