Competent Cells for Routine Cloning/Subcloning
Chemically competent and high transformation efficiency cells that do not require an electroporator.
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Which Competent Cell Strain is Right for You?
| Economical; for routine subcloning | Mid-range transformation efficiency | High-efficiency, best all around | Highest efficiency, ideal for low starting DNA | High efficiency, fast growing | |||||
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| Transformation Efficiency of pUC19 | >1x106 cfu/ug | >1x108 cfu/ug | >1x109 cfu/ug | >5x109 cfu/ug (highest) |
>1x109 cfu/ug | ||||
| One Shot® format | |
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| T1 & T5 Phage resistant (ton A) | |
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| Time to pick colonies | Overnight | Overnight | Overnight | Overnight | Same day | ||||
| Time to purify plasmid DNA after inoculation | Overnight | Overnight | Overnight | Overnight | As soon as 4 hrs | ||||
| Blue/White Screening | |
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| Efficiently transform unmethylated DNA (hsd R) | |
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| Efficiently transform methylated DNA (mcr A) | |
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| Generate ssDNA (Contains F' Episome) | |
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| Kit contains | 4 tubes x 500 µL | 5 tubes x 200 µL | 20 tubes x 50 µL | 20 tubes x 50 µL | 20 tubes x 50 µL | ||||
| Price / 50 µL Reaction | $2.03 (40 reactions) | $9.70 (20 reactions) | $18.33 - $23.40 | $19.05 (20 reactions) | $18.90 (20 reactions) | ||||
| Order Now | Order Now | Order Now | Order Now | Order Now |
Note: DH5α™, TOP10, OmniMAX,™and Mach1 competent cells have: the lacZ∆ M15 for blue/white color screening of recombinants, the recA mutation to minimize non-specific recombination in cloned DNA, and endA1 mutation for increased plasmid yield and quality.
