Competent Cells for Routine Cloning/Subcloning
Chemically competent and high transformation efficiency cells that do not require an electroporator.
Which Competent Cell Strain is Right for You?
|Economical; for routine subcloning||Mid-range transformation efficiency||High-efficiency, best all around||Highest efficiency, ideal for low starting DNA||High efficiency, fast growing|
|Transformation Efficiency of pUC19||>1x106 cfu/ug||>1x108 cfu/ug||>1x109 cfu/ug||>5x109 cfu/ug
|One Shot® format|
|T1 & T5 Phage resistant (ton A)|
|Time to pick colonies||Overnight||Overnight||Overnight||Overnight||Same day|
|Time to purify plasmid DNA after inoculation||Overnight||Overnight||Overnight||Overnight||As soon as 4 hrs|
|Efficiently transform unmethylated DNA (hsd R)|
|Efficiently transform methylated DNA (mcr A)|
|Generate ssDNA (Contains F' Episome)|
|Kit contains||4 tubes x 500 µL||5 tubes x 200 µL||20 tubes x 50 µL||20 tubes x 50 µL||20 tubes x 50 µL|
|Price / 50 µL Reaction||$2.03 (40 reactions)||$9.70 (20 reactions)||$18.33 - $23.40||$19.05 (20 reactions)||$18.90 (20 reactions)|
|Order Now||Order Now||Order Now||Order Now||Order Now|
Note: DH5α™, TOP10, OmniMAX,™and Mach1 competent cells have: the lacZ∆ M15 for blue/white color screening of recombinants, the recA mutation to minimize non-specific recombination in cloned DNA, and endA1 mutation for increased plasmid yield and quality.