Competent Cells for Genomic & cDNA Library Construction
OminMAX™ and DH10B™ competent cells have eliminated mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries. |
Which Competent Cell Strain is Right for You?
| gDNA & plasmid rescue | Inverted & direct repeats & retroviral uses | High efficiency | Highest efficiency; for large inserts | Highest chemically competent efficiency | |||||
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| Transformation Efficiency of pUC19 | >1x1010 cfu/µg | >5x109 cfu/µg | >1x1010 cfu/µg | >3x1010 cfu/µg (highest) |
>5x109 cfu/µg | ||||
| Plasmid size | >20 kb | >100 kb | >20 kb | >100 kb | >20kb | ||||
| Competent cell type | Electrocompetent | Electrocompetent | Electrocompetent | Electrocompetent | Chemically Competent | ||||
| T1 and T5 Phage resistant (ton A) |  |
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| Generate ssDNA (Contains F' Episome) | |
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| Cloning unstable inserts | |
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| One Shot® format | |
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| Blue/White Screening | |
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| Kit contains | 5 tubes x 100 µl | 5 tubes x 100 µl | 5 tubes x 100 µl | 5 tubes x 100 µl | 20 tubes x 50 µl | ||||
| Price per transformation | $10.48 (20 µl/reaction) |
$10.76 (20 µl/reaction) |
$10.76 (20 µl/reaction) |
$11.88 (20 µl/reaction) |
$19.05 (50 µl/reaction) |
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| Order Now | Order Now | Order Now | Order Now | Order Now |
Note: Both the TOP10 and Mach1 competent cells have: the lacZ∆ M15 for blue/white color screening of recombinants, the recA mutation to minimize non-specific recombination in cloned DNA, endA1 mutation for increased plasmid yield and quality, and the hsdR mutation for efficient transformation of unmethylated DNA from PCR amplifications.
