Competent Cells for High Throughput Cloning
MultiShot™ StripWell competent cells are packaged in versatile stripwell tubes (50-µl aliquots), making them ideal for efficient High-throughput (HTP) as well as medium-throughput cloning applications.
Which Competent Cell Strain is Right for You?
|96-well plate format for HTP cloning||Versatile stripwell tubes for medium to HTP applications||Highest efficiency in versatile stripwell format|
|Format||96-well plates for automated applications||12 strips of 8 tubes in a 96-well plate format||12 strips of 8 tubes in a 96-well plate format|
|Flexibility||Use as needed||8 reaction combinations|
|Volume / well||15 µL||50 µL||50 µL|
|Kit size||5 plates||1 plate||1 plate|
|Transformation efficiency||>1x108 cfu/µg||>1x108 cfu/µg||>1x109 cfu/µg
|T1 and T5 Phage resistant (ton A)|
|Time to pick colonies||Overnight||Overnight||Same day|
|Time to purify plasmid DNA after inoculation||Overnight||Overnight||As soon as 4 hrs|
|mcrA for efficient transformation of methylated DNA from genomic preparations|
|Price / transformation||$6.34||$6.99 - $7.70||$8.04|
|Order Now||Order Now||Order Now|
Note: Both the TOP10 and Mach1 competent cells have: the lacZ∆ M15 for blue/white color screening of recombinants, the recA mutation to minimize non-specific recombination in cloned DNA, endA1 mutation for increased plasmid yield and quality, and the hsdR mutation for efficient transformation of unmethylated DNA from PCR amplifications.