Competent Cells for High Throughput Cloning
MultiShot™ StripWell competent cells are packaged in versatile stripwell tubes (50-µl aliquots), making them ideal for efficient High-throughput (HTP) as well as medium-throughput cloning applications. |
Which Competent Cell Strain is Right for You?
| 96-well plate format for HTP cloning | Versatile stripwell tubes for medium to HTP applications | Highest efficiency in versatile stripwell format | |||
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| Format | 96-well plates for automated applications | 12 strips of 8 tubes in a 96-well plate format | 12 strips of 8 tubes in a 96-well plate format | ||
| Flexibility | Use as needed | 8 reaction combinations | |||
| Volume / well | 15 µL | 50 µL | 50 µL | ||
| Kit size | 5 plates | 1 plate | 1 plate | ||
| Transformation efficiency | >1x108 cfu/µg | >1x108 cfu/µg | >1x109 cfu/µg (highest) |
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| T1 and T5 Phage resistant (ton A) | |
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| Time to pick colonies | Overnight | Overnight | Same day | ||
| Time to purify plasmid DNA after inoculation | Overnight | Overnight | As soon as 4 hrs | ||
| mcrA for efficient transformation of methylated DNA from genomic preparations | |
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| Price / transformation | $6.34 | $6.99 - $7.70 | $8.04 | ||
| Order Now | Order Now | Order Now |
Note: Both the TOP10 and Mach1 competent cells have: the lacZ∆ M15 for blue/white color screening of recombinants, the recA mutation to minimize non-specific recombination in cloned DNA, endA1 mutation for increased plasmid yield and quality, and the hsdR mutation for efficient transformation of unmethylated DNA from PCR amplifications.
