Competent Cells for Protein Expression

These competent cells are optimized for protein expression from T7 promoters. All are induced with IPTG or L-arabinose and come in the convenient One Shot® format, allowing for transformation and recovery in a single tube.

Which Competent Cell Strain is Right for You?

  High level expression of recombinant proteins Highest protein expression from low copy plasmids Highest protein expression from high copy plasmids Optimized or ideal for toxic proteins
Increased mRNA stability (mutated rne gene)   yes yes
Expression of known toxic genes     yes
Top Seller yes  
Best expression vector format pET-based expression vector (low copy number T7-based plasmids) pET-based expression vector (low copy number T7-based plasmids) pCR® T7-based expression vector (high copy number, T7-based plasmids) ANY
T7-based expression vector
Inducing agent IPTG IPTG IPTG Arabinose or Arabinose & IPTG
One Shot® format One Shot® One Shot® One Shot® One Shot®
Kit contains 20 tubes x 50 µL 20 tubes x 50 µL 20 tubes x 50 µL 20 tubes x 50 µL
Price per transformation $13.40 – $17.90 $17.50 $17.75 $17.15
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Note: All cells optimized for protein expression from T7 promoters. All are induced with IPTG or L-arabinose and come in the convenient One Shot® format, allowing for transformation and recovery in a single tube.