DNA End Repair Mix

DNA End Repair Mix offers an easy, one step process for creating blunt ends in sheared, sonicated, or restriction-cut DNA and PCR fragments for efficient ligation into blunt end cloning vectors.  

DNA End Repair Mix allows you to:

  • Generate blunt ends in DNA and PCR fragments (A-tail)
  • Repair DNA with 5’ or 3’ overhangs in 30 minutes at 37 °C

A Simple Way to Make Blunt Ends

 Click to Enlarge

With DNA End Repair Mix, DNA with 5’ or 3’ overhangs is converted to 5’ phosphorylated blunt end DNA in 30 minutes at 37 °C. Once DNA has been repaired, the enzymes in DNA End Repair Mix can be column purified or phenol:chloroform extracted thus preventing similar fill in or repair of DNA to be added to the mix.

DNA End Repair Mix enzymes are premixed to minimize pipetting. T4 DNA polymerase, with both 3’ -> 5’ exonuclease activity and 5’ -> 3’ polymerase activity is optimized for filling or cutting 5’ or 3’ overhangs. T4 polynucleotide kinase is included for phosphorylation of 5’ ends. DNA End Repair Mix is optimized for blunting up to 5 μg of DNA at a time.
${comergentUrl}

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Select Language