Genomic DNA and cDNA Libraries

A DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study. There are basically two kinds of libraries: genomic DNA and cDNA libraries. Genomic DNA libraries contain large fragments of DNA in either bacteriophages or bacterial or P1-derived artificial chromosomes (BACs and. PACs). cDNA libraries are made with cloned, reverse-transcribed mRNA, and therefore lack DNA sequences corresponding to genomic regions that are not expressed, such as introns and 5′ and 3′ noncoding regions. cDNA libraries generally contain much smaller fragments than genomic DNA libraries, and are usually cloned into plasmid vectors.

Life Technologies provides innovative Invitrogen™ products for the construction of full-length cDNA libraries from your source material, as well as premade libraries from a number of tissue sources including human, mouse, rat, C. elegans, and Arabidopsis thaliana.

Genomic DNA Libraries

Our genomic DNA library collection consists of BAC/PAC genomic DNA clone resources from the California Institute of Technology and Roswell Park Cancer Institute.

cDNA Library Construction Kits

Construct high-quality full-length cDNA libraries using SuperScript® reverse transcriptase and Gateway® recombination cloning (no restriction enzyme digestion needed), or use standard restriction enzyme–based cloning.

Premade cDNA Libraries

Premade cDNA libraries are ideal for isolating novel and published cDNAs, PCR amplification of target sequences, and expression in eukaryotic cells. Premade cDNA libraries are available from human, human stem cell, mouse, rat, C. elegans, and Arabidopsis tissues.

RACE Kits

Kits for Rapid Amplification of cDNA Ends (RACE) to amplify uncloned nucleic acid sequences at the 3′ or 5′ ends of mRNAs.

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