Genomic DNA and cDNA Libraries
A DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study. There are basically two kinds of libraries: genomic DNA and cDNA libraries. Genomic DNA libraries contain large fragments of DNA in either bacteriophages or bacterial or P1-derived artificial chromosomes (BACs and. PACs). cDNA libraries are made with cloned, reverse-transcribed mRNA, and therefore lack DNA sequences corresponding to genomic regions that are not expressed, such as introns and 5′ and 3′ noncoding regions. cDNA libraries generally contain much smaller fragments than genomic DNA libraries, and are usually cloned into plasmid vectors.
Genomic DNA Libraries
Our genomic DNA library collection consists of BAC/PAC genomic DNA clone resources from the California Institute of Technology and Roswell Park Cancer Institute.
- View all library collections
cDNA Library Construction Kits
Construct high-quality full-length cDNA libraries using SuperScript® reverse transcriptase and Gateway® recombination cloning (no restriction enzyme digestion needed), or use standard restriction enzyme–based cloning.
- Learn more about the SuperScript® Full-Length cDNA Library Construction Kit, the commercially available kit that includes a 5′ CAP-mRNA–purification step to help ensure that your cDNA library is full-length.
- Learn more about the CloneMiner™ II cDNA Library Construction Kit
- Order cDNA Library Construction Kits
Premade cDNA Libraries
Premade cDNA libraries are ideal for isolating novel and published cDNAs, PCR amplification of target sequences, and expression in eukaryotic cells. Premade cDNA libraries are available from human, human stem cell, mouse, rat, C. elegans, and Arabidopsis tissues.