CloneMiner™ II cDNA Library Construction Kit
 | CloneMiner™ II cDNA Library Construction Kits enable rapid construction of highly representative cDNA libraries. Gateway® recombination cloning avoids restriction digestion and inefficient ligation reactions, making library construction faster and offering more complete cDNA representation. The high efficiency SuperScript® III reverse transcriptase is included, enhancing the possibility that users may discover previously unobtainable, fully intact clones. |
CloneMiner™ II cDNA Library Construction Kits benefits include:
- High primary titers increase the probability of cloning rare transcripts
- Large average insert sizes
- High percentage of full-length genes
- Highly efficient cloning of cDNA into Gateway® destination vectors
How is This Kit Different From the Original CloneMiner™ Kit?
- The new kit incorporates a simplified protocol
- To ensure high cDNA yields, SuperScript® II Reverse Transcriptase (RT) has been replaced by SuperScript® III Reverse Transcriptase
- To enable reaction setup with fewer pipetting steps, Gateway® BP Clonase® Enzyme Mix has been replaced by Gateway® BP Clonase® II Enzyme Mix, which contains enzymes and buffer in a single mix
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Contents and Storage:
CloneMiner™ II Kits include SuperScript® III RT, Gateway® vectors, reagents for cDNA library construction and ElectroMax™ DH10B™ T1-Phage-resistant Competent Cells.
Sufficient reagents are supplied for 5 cDNA library construction reactions.
Sufficient reagents are supplied for 5 cDNA library construction reactions.
