cDNA Library Construction Kits

Construct high quality full length cDNA libraries using SuperScript® reverse transcriptase combined with Gateway® cloning for uncut libraries (no use of restriction digestion). Or use standard restriction enzyme cloning.

Superscript® Full Length cDNA Library Construction Kit

The SuperScript® Full-Length cDNA Library Construction Kit has been developed for generating full-length cDNA libraries. The kit incorporates an antibody-based enrichment step in a simple, robust protocol, and utilizes a 5-CAP antibody capped to magnetic beads to prevent truncated mRNA from being reverse-transcribed during cDNA synthesis.  The only kit in the market with a step to purify the 5’ CAP-mRNA to ensure your cDNA library is full length!

CloneMiner™ II cDNA Library Construction Kit

The CloneMiner™ II cDNA Library Construction Kit is a second generation CloneMiner™ kit that enables rapid construction of highly representative cDNA libraries without restriction enzyme cloning. This innovative library construction technology combines SuperScript® III Reverse Transcriptase with Gateway® cloning technology, resulting in the discovery of previously unobtainable, full-length clones. The kit also avoids the use of time-consuming, inefficient ligation reactions, making library construction faster and offering better representation.

SuperScript® Plasmid System for cDNA Synthesis with Gateway™ Technology

The SuperScript® Plasmid System is designed for synthesis of double-stranded cDNA from a purified mRNA population. The cDNA is suitable for directional cloning into the pSPORT1 vector or the pCMV•SPORT6 plasmid vector for subsequent generation of cDNA libraries and use of Gateway® Technology.

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