SuperScript® hESC Cell Line History and Characteristics

  • The stem cell lines BG01v, BG01, BG02, and BG03 were used to make the SuperScript® hESC Stem Cell cDNA Libraries.
  • The hESC cell lines were cultured in hESC medium conditioned by mitomycin C-inactivated mouse embryonic fibroblast cells (MEF) in a feeder free fashion 1,2 .
  • All hESC lines are pluripotent.  The Immuno markers and other cell line characteristics 3,4,5,6 are summarized in Table 1.

Immuno markers and characteristics of hESC cell lines


Table 1. Characteristics of cDNA library hESC cell lines
 BG01VBG01BG02BG03
SourceHuman blastocystHuman blastocystHuman blastocystHuman blastocyst
Passage67653518
KaryotypeAbnormal 48XY/12+/17+Normal           46XYNormal             46XYNormal            46XX
GenderMaleMaleMaleFemale
PluripotentYesYesYesYes
Immuno Markers:
SSEA-1----
SSEA-3++++
SSEA-4++++
TRA 1-60++++
TRA 1-81++++
Oct-4++++
Alkaline phosphatase++++

References

  1. Brimble, S.N., et al.. 2004 Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001.  Stem Cells Dev. 13(6):585-97
  2. Wang, L,et al. 2007 Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling. Blood doi:10.1182/blood-2007-03-082586
  3. National Institutes of Health 2007. Stem Cell Information. http://stemcells.nih.gov/staticresources/research/registry/PDFs/BresaGen.pdf
  4. Josephson, R., et. al. 2007  Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Stem Cells  25; 437-446
  5. Liu, Y., et. al. 2006 Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S. Federal government approved hESC lines.  BMC Dev Biol. 6 (20):
  6. Zeng, X., et. al. 2004 Properties of Pluripotent Human Embryonic Stem Cells BG01 and BG02. Stem Cells 22:292-312