SuperScript® hEC cDNA Libraries: Library Construction and Library Statistics
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- SuperScript® hEC cDNA libraries were constructed using SuperScript® III reverse transcriptase for first-strand synthesis to generate high percentages of full-length and high-yield cDNA. For library cloning, double strand cDNA was digested with Not1 and directionally ligated into the Gateway®-compatible library vector pCMV-SPORT6.1.
- The resulting standard libraries for the hEC cell lines were quality tested for the average insert size, the percent recombinants, and the titer (cfu/ml) of the library (Table 1).
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SuperScript® hEC cDNA library statistics:
Table 1. Specification and QC data of hEC cDNA libraries | | Specification | cDNA Library Statistics |
| Cell Line Name | | NTera2 | 2102Ep |
| Average Insert Size | ≥ 1.2 kb | 1.8 kb | 2.0 kb |
| % Recombinants | ≥ 87% | 87% | 96% |
| Titer (cfu/ml) | ≥ 1x10e9 | 1.3x10e9 | 6.8x10e9 |
A1030001,A1030101,A1030201,A1030301,A1030401,A1030501
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