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Cloning

Vector NTI Advance™ Software—The Ultimate Gateway® Cloning Design Tool

Tens of thousands of researchers use Vector NTI Advance™ sequence analysis software because it helps save time and effort when designing cloning experiments. And with a built-in cloning software wizard that supports the latest advances in Gateway® Technology, including MultiSite Gateway® Pro, cloning has never been easier.

Imagine having cloning software that can:

  • Guide you step-by-step through the cloning process (Figure 1)
  • Design primers with the right att sites and seamlessly order the primers online (Figure 2)
  • Create graphical maps of recombinants automatically (Figure 3)
  • Enable you to easily perform 2-, 3-, and 4-fragment cloning using MultiSite Gateway® Pro
  • Simultaneously clone multiple fragments into multiple cloning vectors
  • Store, manage, and track all subclones and lineages, Vector NTI Advance™ provides all this, along with dozens of other sequence analysis tools, in one fully integrated cloning software application.

Invitrogen strongly recommends the use of Vector NTI Advance™ 10.3 and later versions to design multiple-fragment cloning strategies using MultiSite Gateway® Pro technology.

Gateway Cloning Wizard Software

Figure 1. Gateway® Cloning Wizard Software
  • The Gateway® Cloning Wizard software guides you through each step of the cloning workflow. This figure shows an example of the LR Reaction Workspace, with a list of Expression Clones to be created in a batch Gateway® cloning experiment.



Figure 2. Primer Design and Ordering
Vector NTI Advance™ automatically generates primers with the correct att recombination sites for BP reactions. This figure shows the primer design dialog box and the Local Database, from which primers can be ordered online either singly or in batch.



Figure 3. Vector NTI® Molecule Viewer
Use the Vector NTI Advance™ automatically generates a graphical map of your Gateway® clones, showing the step-by-step cloning protocol, and the “parent-descendant” relationships between molecules.