Electrocompetent Cells
| Electrocompetent cells work using the electroporation process. Electrical pulses created pores that allows genetic material to permeate the bacterial membrane. Invitrogen offers a variety of electrocompetent E. coli cells to reliably clone your DNA with high efficiency. The convenient packaging formats and a variety of electroporation cuvettes will fulfill all of your electrocompetent cell transformation requirements. |
Electroporation Cuvettes
 | Choose the right electroporation cuvette for your strain. Available in a variety of sizes.
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Electrocompetent cells available from Invitrogen
Cell Strain | Description | Application | Transformation Efficiency |
|---|---|---|---|
MegaX DH10B™ T1R Electrocomp™ Cells | MegaX DH10B™ T1R Electrocomp™ cells are the highest-efficiency electrocompetent cells. See additional details below. | Ideal for highly demanding cloning and library construction applications | >3 x 1010 cfu/μg |
| ElectroMAX™ DH10B™ T1 R Cells | High efficiency cells derived from DH10B™ strain. tonA genotype for T1 and T5 phage resistance. | Efficient cloning of prokaryotic and eukaryotic genomic DNA. Efficient plasmid rescue from eukaryotic genomes. | >1 × 1010 cfu/μg |
| ElectroMAX™DH10B™ Cells | Derived from the DH10B™ strain in a high efficiency electrocompetent format. Also available in bulk format. | Efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. | >1 × 1010 cfu/μg |
| ElectroMAX™ DH5α-E™ Cells | Derived from the DH5α™ strain. Suitable for transformation by electroporation. | Procedures requiring high transformation efficiencies, such as cDNA library generation or with limited input DNA. | >1 × 1010 cfu/μg |
| One Shot® TOP10 Electropcomp™ Cells | Genetically similar to the DH10B™ strain. Available in single-use One Shot® format. | Used for general cloning applications. Found in many TOPO® cloning and expression kits. | >1 × 1010 cfu/μg |
| TOP10 Electropcomp™ Cells | Genetically similar to the reliable DH10B™ strain. | Used for general cloning applications. Found in many TOPO® cloning and expression kits. | >1 × 1010 cfu/μg |
| TOP10F’ Electropcomp™ Cells | Genetically similar to the reliable DH10B™ strain with the F’ genotype. | Used for general cloning applications. | >1 × 1010 cfu/μg |
| ElectroMAX™ DH12S™ Cells | A derivative of DH10B™ are suitable for transformation by electroporation. | A unique genotype to support production of exceptionally clean ssDNA. | >1 × 1010 cfu/μg |
| ElectroMAX™ Stbl4™ Competent Cells | Suitable for transformation by electroporation. Specifically designed for cloning unstable inserts. | >5 × 109 cfu/μg |
Highest-efficiency electrocompetent cells
MegaX DH10B™ T1R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available (Figure 1), with a guaranteed three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC control DNA). They are ideal for highly demanding cloning and library construction applications. MegaX cells have the same genotype as the widely used DH10B™ T1R strain, including tonA to prevent T1 and T5 lytic phage infection and safeguard your valuable clones and libraries; however, they are manufactured using an improved process that results in a significantly higher transformation efficiency. Available in the bulk Electrocomp™ format.
 | Figure 1: MegaX DH10B™ T1R Electrocomp™ cells consistently outperform the competition. L: E.cloni® 10G SUPREME cells (Lucigen); G:GC10™ Thunderbolt™ cells (GeneChoice); S: ElectroTen-Blue® electroporation-competent cells (Stratagene). |
