Competent Cells for Specialized Applications
Competent Cells needed for specialized applications like cloning unstable DNA, making single stranded DNA, Gateway® compatibility, and more. |
Competent cells for specialized applications
| Cell Strain | Description | Application | Transformation Efficiency | |
|---|---|---|---|---|
| Cloning Unstable DNA | ||||
| One Shot® Stbl3™ Chemically Competent Cells | Designed for cloning direct repeats found in lentiviral expression vectors. Available in bulk format. | Cloning unstable DNA | >1 × 108 cfu/μg | |
| MAX Efficiency® Stbl2™ Chemically Competent Cells | High-efficiency chemically competent cells specifically designed for cloning unstable inserts. Available in bulk format. | Cloning unstable DNA | >1 × 109 cfu/μg | |
| ElectroMAX™ Stbl4™ Electrocomp™ Cells | Specifically designed for cloning unstable inserts. Available in bulk format. | Used for applications requiring high transformation efficiencies, such as generating cDNA and genomic libraries. | >5 × 109 cfu/μg | |
| Single-Stranded DNA(ssDNA) production | ||||
| ElectroMAX™ DH12S™ Electrocomp Cells | Carries the F´ episome and endA1 genotype, since double-stranded DNA is degraded in endA+ strains. Available in bulk format. | Can be used for ssDNA production for use in DNA sequencing, preparation of strand-specific probes, in vitro mutagenesis and subtraction library applications. | >1 × 1010 cfu/μg | |
| Propagating unmethylated DNA | ||||
| One Shot® INV110 Cells | INV110 Cells are dam/dcm-deficient and endA1 to ensure high-quality miniprep DNA.Available in the convenient single-use One Shot® format. | For applications that require the production of unmethylated DNA such as the need to perform certain restriction enzyme digestions. | >1 × 106 cfu/μg | |
| Propagating vectors with the ccdB gene | ||||
| One Shot® ccdB Survival 2 T1R Cells | Suitable for propagation of plasmids containing the ccdB gene. Available in the convenient single-use One Shot® format. | Designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. | >1 × 109 cfu/μg | |
| MAX Efficiency® DH10Bac™ Chemically Competent Cells | Contains a parent bacmid that recombines with the donor plasmid, pFastBac™, to create an expression bacmid containing the gene of interest.Available in bulk format. | Used for production of a recombinant bacmid in the Bac-to-Bac® Baculovirus Expression System. | >1 × 108 cfu/μg | |
