GeneArt® Seamless Cloning and Assembly
 | GeneArt® Seamless Cloning and Assembly enables cloning of up to 4 DNA fragments simultaneously into virtually any linearized vector in 30 minutes, without extra DNA sequences, restriction endonucleases or ligation. |
GeneArt® Seamless Cloning & Assembly
The GeneArt® Seamless Cloning and Assembly Kit enables cloning of DNA fragments into virtually any linearized vector, without extra sequences, or restriction endonuclease digestion and ligation. The proprietary enzyme mix recognizes and precisely assembles the DNA fragments sharing a 15-base pair (bp) end homology created by PCR amplification in a simple 30 minute room temperature reaction. The DNA Oligo Designer guides users through experimental design.
) | This figure outlines the GeneArt® Seamless Cloning and Assembly protocol for assembling up to four DNA fragments and a vector simultaneously and in precise order. |
Leader in Multiple Fragment Assembly Efficiency
) | The GeneArt® Seamless Cloning and Assembly Kit delivers high cloning efficiencies across a wide range of fragment sizes and numbers. A comparative analysis against competitor 'I' reveals the advantage of using GeneArt® Seamless Cloning and Assembly when 3 or more DNA fragments are cloned simultaneously. |
Reliable and Easy to Use
) | GeneArt® Seamless Cloning and Assembly is an easy and reliable method for routine cloning needs. Three scientists assembled four 1 kbp DNA fragments following the GeneArt® Seamless Cloning and Assembly protocol with an average cloning efficiency of 95.8%. |
Achieving Optimal Cloning Efficiency
| The GeneArt® Seamless Cloning and Assembly Kit provides high cloning efficiency across a wide range of fragment sizes and fragment numbers. Using the AccuPrime® Pfx SuperMix to amplify the inserts and/or vector can significantly increase the cloning efficiency in a multifragment assembly as compared to the Platinum® PCR SuperMix High Fidelity. |
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Design PCR primers to create end-terminal homology between your adjacent DNA fragments
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Genetic Engineering and Biotechnology News article
Volume 30, Number 18 October 15, 2010
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