TOPO® PCR Cloning With Mach1™ Competent Cells

TOPO® PCR Cloning
Invitrogen's TOPO® cloning kits with our Mach1™-T1R E. coli strain make cloning fast and simple.  Mach1™ cells deliver robust growth for miniprep analysis in just four hours, saving up to 2 days compared to restriction enzyme and ligation cloning.

Simple 3-Step Protocol

We offer a suite of TOPO® cloning kits with Mach1™-T1R cells for basic cloning of Taq-amplified fragments, blunt-ended fragments, sequencing, long-fragment PCR cloning, and entry into the Gateway® system.  Simply add your PCR product (blunt-ended fragment or amplified using Taq polymerase) to the appropriate vector, incubate for 5 minutes and transform into our fast growing Mach1™ competent cells.

Mach1™-T1R E. coli

Fast Growing Cell Saves up to 2 Days

Mach1™-T1R E. coli  Invitrogen's Mach1™-T1R E. coli achieve sufficient growth for miniprep analysis from an overnight colony in just four hours (Figure 1) compared to conventional strains which require an overnight incubation.


Mach1™- T1R cells offer several advantages:

  • Reduced occurrence of unwanted recombination
  • Resistance to T1 and T5 phages
  • Clean DNA preparations
  • Blue/white screening for easy, visual recombinant clone identification

Cloning Blunt Ended Fragments

ZeroBlunt® TOPO® PCR Cloning Kits with Mach1™ Competent Cells accelerate and simplify cloning of PCR fragments generated by a proofreading polymerase such as Pfx, Pfx50 and AccuPrime Pfx.


Order the best ZeroBlunt® TOPO® kit with Mach1™ cells by your application:

 
 

Cloning Taq-amplified Products

Fast, reliable, and direct, TOPO® TA Cloning with Mach1™ cells gets the right clone in your hands sooner, freeing up your time to answer more important questions.


Order the best TOPO® TA Cloning® Kit with Mach1™ cells by your application:

 

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