TOPO® PCR Cloning With Mach1™ Competent Cells
Invitrogen's TOPO® cloning kits with our Mach1™-T1R E. coli strain make cloning fast and simple. Mach1™ cells deliver robust growth for miniprep analysis in just four hours, saving up to 2 days compared to restriction enzyme and ligation cloning.
Simple 3-Step Protocol
We offer a suite of TOPO® cloning kits with Mach1™-T1R cells for basic cloning of Taq-amplified fragments, blunt-ended fragments, sequencing, long-fragment PCR cloning, and entry into the Gateway® system. Simply add your PCR product (blunt-ended fragment or amplified using Taq polymerase) to the appropriate vector, incubate for 5 minutes and transform into our fast growing Mach1™ competent cells.
Fast Growing Cell Saves up to 2 Days
|Invitrogen's Mach1™-T1R E. coli achieve sufficient growth for miniprep analysis from an overnight colony in just four hours (Figure 1) compared to conventional strains which require an overnight incubation.|
Cloning Blunt Ended Fragments
ZeroBlunt® TOPO® PCR Cloning Kits with Mach1™ Competent Cells accelerate and simplify cloning of PCR fragments generated by a proofreading polymerase such as Pfx, Pfx50 and AccuPrime Pfx.
Order the best ZeroBlunt® TOPO® kit with Mach1™ cells by your application:
Cloning Taq-amplified Products
Fast, reliable, and direct, TOPO® TA Cloning with Mach1™ cells gets the right clone in your hands sooner, freeing up your time to answer more important questions.