Cloning for Sequencing Applications
|The TOPO® cloning kits for sequencing allow fast cloning and streamlined sequencing of PCR products. These kits contain TOPO® cloning vectors with a minimized multiple cloning site that positions the T7 and T3 priming sites only 33 bp away from the PCR product insertion site (Figure 1). This means you’ll sequence more of your insert and less of the vector. |
Choice of Vector
|The pCR®4-TOPO® vector supplied in the TOPO® TA Cloning® Kit for Sequencing has 3´-T overhangs for cloning Taq-amplified PCR products. The pCR®4Blunt-TOPO® vector supplied in the Zero Blunt® TOPO® PCR Cloning Kit for Sequencing has blunt ends for cloning PCR products amplified with proofreading polymerases. Convenient features of these sequencing vectors include:|
Figure 1: pCR® 4-TOPO® cloning vectors for sequencing.