We offer several TOPO® cloning kits that allow direct access to our Gateway® expression system. Our new pCR®8/GW/TOPO® vector (Figure A) offers streamlined sequence analysis so you sequence more of your insert and less of the vector. With our patented att sites, the pCR®8 vector serves as a convenient entry vector to the Gateway® system, allowing you to easily access a variety of expression vectors without tedious subcloning steps, and saving you valuable research time.
The pENTR™ vector series (Figure B) provides Directional TOPO® cloning, capturing your insert in the correct orientation for protein expression. Additionally, our new pENTR™/TEV/D-TOPO® vector has a tobacco etch virus (TEV) recognition site for efficient cleavage of any N-terminal tag and 5’ att site after recombination with the destination vector. pcDNA™/V5-GW/D-TOPO® vectors (Figure C) are also available for high-level mammalian expression followed by possible analysis in other systems.