Access to Gateway® vectors via TOPO® Cloning
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TOPO® cloning into the Gateway® system
We offer several TOPO® cloning kits that allow direct access to our Gateway® expression system. Our new pCR®8/GW/TOPO® vector (Figure A) offers streamlined sequence analysis so you sequence more of your insert and less of the vector. With our patented att sites, the pCR®8 vector serves as a convenient entry vector to the Gateway® system, allowing you to easily access a variety of expression vectors without tedious subcloning steps, and saving you valuable research time.
The pENTR™ vector series (Figure B) provides Directional TOPO® cloning, capturing your insert in the correct orientation for protein expression. Additionally, our new pENTR™/TEV/D-TOPO® vector has a tobacco etch virus (TEV) recognition site for efficient cleavage of any N-terminal tag and 5’ att site after recombination with the destination vector. pcDNA™/V5-GW/D-TOPO® vectors (Figure C) are also available for high-level mammalian expression followed by possible analysis in other systems.
The pENTR™ vector series (Figure B) provides Directional TOPO® cloning, capturing your insert in the correct orientation for protein expression. Additionally, our new pENTR™/TEV/D-TOPO® vector has a tobacco etch virus (TEV) recognition site for efficient cleavage of any N-terminal tag and 5’ att site after recombination with the destination vector. pcDNA™/V5-GW/D-TOPO® vectors (Figure C) are also available for high-level mammalian expression followed by possible analysis in other systems.
Access to multiple applications
| Only Gateway® technology gives you ultimate access to multiple expression options. Rapidly transfer your gene and express it in as many systems as you choose. Take the first step towards accessing all of your expression needs by simply cloning your gene into a Gateway® entry vector. Whether you prefer TOPO®, PCR, or restriction cloning, we have a Gateway® entry vector for you.
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