| Application of cloned PCR product | Product | Type of TOPO® cloning | Advantages | One Shot® Competent Cells | Cat. no. | |
| General subcloning | TOPO® TA Cloning® Kit | TA | Get your clone 95% efficiency Selection of competent cells for high speed, electroporation, or routine cloning | Mach1-T1R | K451020 |  |
| TOP10 | K450001 | |
| TOP10F’ | K455001 | |
| TOP10 Electrocomp™ | K456001 | |
| MAX Efficiency® DH5α™-T1 Phage-Resistant | K452001 | |
| Zero Blunt® TOPO® PCR Cloning Kit | Blunt | Unique technology minimizes background Get your clone 95% efficiency Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convinient Selection of competent cells for high speed, electroporation, or routine cloning | Mach1™-T1R | K283020 | |
| TOP10 | K280020 | |
| TOP10 Electrocomp™ | K286020 | |
| MAX Efficiency® DH5α™-T1 Phage-Resistant | K282020 | |
| Long PCR fragments | TOPO® XL PCR Cloning Kit | TA | Combining TOPO®, ZeroBackground™ and Crystal Violet technologies makes for easy cloning of large fragments 3-10 kb. Unique technology to minimize background | Mach1™-T1R | K703020 | |
| TOP10 | K475010 | |
| TOP10 Electrocomp™ | K470010 | |
| In vitro transcription | TOPO® TA Cloning® Kit Dual Promoter | TA | Get your clone 95% efficiency Dual T7 and SP6 priming sites for in vitro transcription | Mach1™-T1R | K461020 | |
| TOP10F’ | K460001 | |
| TOP10 Electrocomp™ | K466001 | |
| MAX Efficiency® DH5α™ -T1 Phage-Resistant | K462001 | |
| Sequencing | TOPO® TA Cloning® Kit for Sequencing (pCR® 4 TOPO® Vector) | TA | Get your clone 95% efficiency Sequence more insert, less vector Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convinient | Mach1™-T1R | K453020 | |
| TOP10 | K457501 | |
| TOP10 Electrocomp™ | K458001 | |
| MAX Efficiency® DH5α™ -T1 Phage-Resistant | K289520 | |
| Zero Blunt® TOPO® PCR Cloning Kit for Sequencing (pCR® 4 Blunt TOPO® Vector) | Blunt | Unique technology minimizes background Get your clone 95% efficiency Sequence more insert, less vector | Mach1™-T1R | K283520 | |
| TOP10 | K287520 | |
| MAX Efficiency® DH5α™-T1 Phage-Resistant | K289520 | |
| TOP10 Electrocomp™ | K288020 | |
| Expression in E. coli | Champion™ pET100 Series Directional TOPO® Expression Kits (pET Directional TOPO® Vector) | Directional | Up to 90% of clones in the correct orientation High-level protein expression using E. coli Convenient purification and detection tags | BL21 Star™(DE3) | K10001 | |
| K10101 | |
| K10201 | |
| K15101 | |
| Expression in mammalian cells | pcDNA™ 3.3 TOPO® TA Cloning® Kit | TA | 3–5 higher expression compared to conventional CMV vectors Ideal for expression of native protein | TOP10 | K830001 | |
| pcDNA™ 3.2/V5/GW/D-TOPO® Cloning Kit | Directional | Greater than 90% of clones in correct orientation CMV promoter delivers high-level expression in mammalian cells C-terminal V5 and 6xHis tags for convenient detection and purification Neomycin selection | TOP10 | K244020 | |
| ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kits (pLenti 7.3 Vector) | TA | Greater than 4X higher expression compared to the original system Efficient TOPO® cloning | Stbl3™ | K495000 | |
| Entry into Gateway® Entry Vectors | pCR®8/GW/TOPO® TA Cloning® Kit | TA | Includes Plasmid miniprep kit Fast growing cells for fast miniprep | Mach1™-T1R | K532000 | |
| TA | Fast growing cells for fast miniprep | Mach1™-T1R | K252020 | |
|
| TOP10 | K250020 | |
| pENTR™/TEV/DTOPO® Cloning Kit | Directional | Fast growing cells for fast miniprep | Mach1™-T1R | K252520 | |
| pENTR™/D-TOPO® Cloning Kit | Directional |
| TOP10 | K240020 | |
| pENTR™/SD/DTOPO® Cloning Kit | Directional |
| TOP10 | K242020 | |
