A 5-minute, room-temperature reaction requires no gel purification or post-PCR modifications and yields up to 95% recombinants. TOPO® cloning kits are available for general subcloning, long PCR fragments, in vitro transcription, and sequencing. They are also available for expression in E. coli, mammalian cells, and entry into the Gateway® expression system. TA Cloning® kits are designed for cloning directly from a PCR reaction using an overnight ligation step.
Which PCR Cloning Method is Right for You?
|Traditional restriction enzyme cloning||One-step cloning with PCR||5 minute cloning with the highest efficiency||Clone once, easily shuttle insert into many vectors||Seamless cloning of multiple fragments|
|Cloning Efficiency||~50%||80-95%||Up to 95%||Up to 99%||90% single fragment, 75% 4 fragments|
|Time Spent on Cloning||Hours||Hours||5 minutes||1 hour||30 minutes|
|Maximum Product Size||Variable||1-3 kb||<5 kb
(<10 kb for XL-TOPO®)
|Up to 5 kb||13-110 kb|
|Time to Clone Multiple Fragments||Days-weeks||Not possible||Not possible||>4 days||1-3 days|
|Maximum # of Fragments||Varies||1||1||4||10|
|Seamless (no extra sequences)|
|Price/Reaction||Varies||$11.55 - $20.20||$13.55 - $37.10||$18.49 - $22.36||$14.95 - $40.30|
|Order Now||Order Now||Order Now||Order Now||Order Now|