Robust, high-efficiency systems and kits to streamline your workflow
Which Site-Directed Mutagenesis System is Right for You?
|Mutagenesis Efficiency||>90% for 1 site||>90% for 1, 2, or 3 sites|
|# of Mutations||Up to 25 nt mutations in 1 site/plasmid||
|Time-to-Results||Typically less than 3 hours for 3 kb plasmid||Typically ~3 hours for 10 kb plasmid or smaller|
|Order Now||Order Now|
GeneArt® Site-Directed Mutagenesis Workflow
|Figure 1: The GeneArt® Site-Directed Mutagenesis kit delivers superior mutagenesis performance with a wide range of vector sizes. A comparative analysis against competitor 'Q' reveals the advantage of using GeneArt® Site-Directed Mutagenesis for a single base pair mutation.|
High Mutagenesis Efficiency
Multiple base mutagenesis is common, and we tested a 12 base substitution, insertion and deletion using a pUC19 plasmid. A random 12 base substitution was carried out within a single mutated primer. Alternatively, a random 12 base oligonucleotide containing a stop codon was inserted into wild type pUC19 plasmid, followed by deletion of the exact 12 bases to restore the wild type plasmid.
Multisite-Directed Mutagenesis Efficiency
|Figure indicates the multisite-directed mutagenesis efficiency of 3 sites of 1 bp or 3 bp each in 5, 10, and 14 kb plasmids. In all cases the mutated sites (1 or 3 bp each) included one insertion, one deletion and one substitution. The performance of GeneArt® Site-Directed Mutagenesis PLUS System was comparable to the latest generation of multisite-directed mutagenesis kits from the competitor.|