Site-Directed Mutagenesis

Mutagenesis

A highly efficient system for site-directed mutagenesis to generate substitutions, deletions, or insertions in virtually any plasmid.
    

  • Change up to 12 bp at a time in plasmids up to 14.5 kb
  • Protocol is complete in less than 3 hours (3 kb plasmid)
  • Mutagenesis efficiency over 90% (3 kb plasmid)

 

Order Now

GeneArt® Site-Directed Mutagenesis Protocol

GeneArt® Site-Directed Mutagenesis utilizes mutagenic oligonucleotide primers to generate mutations. The mutagenesis protocol is streamlined by combining DNA methylation and amplification steps into a single reaction, and eliminating post-mutagenesis digestion and purification steps.

 Figure 1:  The GeneArt® Site-Directed Mutagenesis protocol.

High Mutagenesis Efficiency

The GeneArt® Site-Directed Mutagenesis kit delivers superior mutagenesis performance with a wide range of vector sizes. A comparative analysis against competitor 'Q' reveals the advantage of using GeneArt® Site-Directed Mutagenesis for a single base pair mutation.

 Multiple base mutagenesis is common, and we tested a 12 base substitution, insertion and deletion using a pUC19 plasmid. A random 12 base substitution was carried out within a single mutated primer. Alternatively, a random 12 base oligonucleotide containing a stop codon was inserted into wild type pUC19 plasmid, followed by deletion of the exact 12 bases to restore the wild type plasmid.

 

 The mutagenesis efficiency for a 12 base substitution, insertion or deletion was above 90%. The performance of GeneArt® Site-Directed Mutagenesis kit was comparable to the latest generation of kits from competitor 'Q'.

Take control of your mutagenesis experiments today!
Change Location Change Location
  • Youtube
  • Twitter
  • Facebook