Gateway® Recombination Cloning Technology Online Seminars

Online Seminars

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Introduction to Gateway® Recombination Cloning

  • Defining Gateway® technology
  • Advantages of Gateway® cloning
  • Ways to enter the Gateway® system
  • How to obtain a Gateway® Expression clone

Protein Expression

  • Description of Destination vectors
  • Examples of Expression clones in Destination vectors
  • Effects of att sites on prokaryotic and mammalian expression
  • How to create your own Destination vector

Creating an Entry clone

  • Options for entering the Gateway® system
  • Defining the BP and LR Clonase™ enzyme reactions
  • Description of Ultimate™ ORF collection
  • Description of Vector NTI Advance™ software for in silico cloning

Cloning multiple fragments into a single vector

  • How to clone up to 4 DNA fragments simultaneously into one destination vector
  • Examples of expression of multiple genes in HeLa cells
  • Example of testing the effects of promoters and regulatory elements on protein expression
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