MultiSite Gateway® Pro Kits

Assemble DNA Fragments Simultaneously assemble 2, 3 or 4 DNA fragments into one vector

MultiSite Gateway® Pro Kits:
  • Simplified cloning of multiple DNA fragments—Gateway® recombination eliminates the use of restriction enzymes and ligases
  • Flexible design—clone up to four DNA elements into any Gateway® Destination vector
  • Reliable gene delivery—avoid uncertain cotransfection by creating one plasmid containing all your DNA elements

Multiple Fragment Assembly Simplified



Figure 1. Overview the MultiSite Gateway® Pro Technology

Figure 2.  An example of using MultiSite Gateway® Pro Technology to study expression of multiple genes in human cells. Entry clones encoding genes for YFP and CFP and the CMV and EF-1α promoters were recombined into pcDNA™ 6.2/V5-PL-DEST (A, C, and E) or into pcDNA™ 6.2/V5-DEST (B and D). The resulting expression clones were transfected into HeLa cells. Expression was verified under a fluorescence microscope. The plasmid pcDNA™ 6.2/V5-PL-DEST is a promoterless derivative of pcDNA™ 6.2/V5-DEST, which carries the CMV promoter.

Easy Experimental design with Vector NTI Advance™ Software

Invitrogen's Vector NTI Advance™ sequence analysis software simplifies designing your primers for Multi-Site Gateway® recombination reactions.  With an user- friendly interface, you select or import the DNA fragment sequences that you wish to clone and choose a destination vector. The Vector NTI Advance™ software generates sequences for PCR primers that amplify your DNA elements, and shows you the vector map and sequences of your Entry Clone(s) as well as the final Expression Clone construct.  It's that simple.

  •  Learn more and download your copy of  Vector NTI Advance™ Software.