Gateway® Recombination Cloning Technology

The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. This traditional method limits your cloning success. For example, certain restriction enzymes cannot be used because they might cut within your gene of interest, truncating the insert and making the gene useless for downstream expression. Additional clean-up steps are needed with this method, you experience low-efficiency recovery of recombinants from cloning large fragments, and you waste time screening colonies to find the clone you need—all of these steps take considerable time and effort and success is not guaranteed.

Amazingly Versatile

In contrast, Gateway® recombination cloning technology circumvents these cloning limitations, enabling you to access virtually any expression system. Gateway® recombination cloning uses a one hour, 99%-efficient, reversible recombination reaction, without using restriction enzymes, ligase, subcloning steps, or screening of countless colonies, thereby saving you time, money, and effort. Widely adopted in the research community with more than 1,500 references since its launch, Gateway® technology makes collaboration across research disciplines easy and convenient and enables access to a multitude of vectors from these research groups for truly multidisciplinary scientific studies. Finally, new advancements such as MultiSite Gateway® Technology makes Invitrogen’s Gateway® cloning the ideal cloning method for protein expression and functional analysis.

Advantages of Gateway® Technology

  • Fast, one hour, room temperature cloning reactions with >99% efficiency deliver the clone you need
  • Maintaining orientation and reading frame without using restriction enzymes or ligation makes expression-ready clones
  • Eliminating re-sequencing ensures consistent results throughout your experiment using the same clone from target identification to validation
  • Shuttling insert DNA from one expression vector to another affords flexibility while simplifying your cloning workflow

 Figure 1 - DNA Fragments
Figure 2. Gateway® technology facilitates cloning of genes, into and back out of, multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone you can then move the DNA fragment into one or more destination vectors simultaneously.

Construct your Gateway® Entry Clone

Start using Gateway® Recombination Cloning Today: Construct your Entry clone
There are various methods to enter the Gateway® platform, including use of TOPO® cloning vectors containing Gateway® att sites, or purchasing an Ultimate™ ORF Clone already inserted into a Gateway® vector.