Gateway® Destination Vectors
 | Expanded Options in Versatile Gateway® Recombination CloningThe Gateway® Cloning Technology provides a rapid and highly efficient route to protein expression, functional analysis and cloning/subcloning of DNA segments. Three new Gateway®-compatible destination vectors are now available to expand your expression options. These vectors contain the Gateway® recombination sites, allowing rapid, efficient transfer of sequences into these new expression systems using site-specific recombination. The release of these vectors increases the variety of applications in which the Gateway® Recombination Cloning Technology can be used. |
High Level Inducible Mammalian Expression
pT-REx™-DEST30 and pT-REx™-DEST31 are part of the T-REx™ system, the first inducible mammalian expression system to use the full-length CMV promoter providing high level, regulated expression. There is no toxicity with T-REx™ because viral transactivators are not used. Easy selection of stable cells using GENETICIN® is possible due to the presence of the Neomycin resistance gene. Additionally, pT-REx™-DEST31 contains a polyhistidine (6xHis) tag allowing for rapid purification on ProBond™ resin or detection with an Anti-His Antibody.
Regulated High-level E.coli Expression
pET™-DEST42 utilizes the T7/lac promoter for regulated, high-level expression in E. coli. The vector has the pBR322 low-copy origin of replication for minimized basal expression level. A C-terminal V5 epitope and polyhistidine (6xHis) tag allow for detection using an Anti-V5 Antibody and an Anti-His Antibody respectively.
NEW Gateway®-Compatible Destination Vectors:
| Vector | Expressed in | Features | Size |
|---|---|---|---|
| pT-REx™-DEST30 | Mammalian | CMV promoter, no viral transactivators, Neomycin resistance | 7544 bp |
| pT-REx™-DEST31 | Mammalian | CMV promoter, no viral transactivators, Neomycin resistance, N-terminal 6X histidine affinity tag | 7559 bp |
| pET™-DEST42 | E. coli | T7 promoter, C-terminal V5 epitope, C-terminal 6X histidine affinity tag | 7440 bp |
