Clonase™ Enzyme












Cloning

Clonase™ Enzymes--mediating the recombination reaction

As explained, the core of Gateway® cloning is the Entry vector. Once the Entry clone is ready, the gene of interested is easily shuttled to a secondary plasmid, the Destination vector. This reaction is mediated by a robust enzyme mixture called LR Clonase®, which contains the necessary protein activity to excise the gene of interest from the Entry clone and integrate it into the Destination vector, which then becomes your expression clone (Figure 6). Reversing this reaction simply entails performing a BP reaction with BP Clonase® enzyme mix. Both LR and BP Clonase® enzyme mixtures are easy-to-use master mix formats ensuring consistency and reliability from reaction to reaction.

Clonase® enzymes versions

  • Gateway® BP Clonase™ Enzyme Mixtures
    Gateway BP Clonase™ enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones. Check out the selection guide below to find the best BP Clonase™  enzyme


    Gateway® LR Clonase™ Enzyme mixes
    Gateway® LR Clonase™ II enzyme mix contains a proprietary blend of Int (Integrase),  IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format.  Check out the selection guide below to find the best LR Clonase™  enzyme

Select the Best Clonase™ Enzyme!

RatingEnzyme FormatAdvantages
BestLR Clonase™ II Plus Enzyme MixSingle mix of enzyme and reaction buffer
  • Highest cloning efficiency for single and multiple fragment cloning
  • Optimized for difficult cloning reactions
  • Works with MultiSite Gateway® Pro Technology
  • Easy to use single mix format ensures enzyme stability
  • Convenient 10 µl reaction set up
Very goodLR Clonase™ II Enzyme MixSingle mix of enzyme and reaction buffer
  • High efficiency for single fragment cloning
  • Convenient master single mix format eliminates pippetting steps and error
  • Easy to use single mix format ensures enzyme stability
  • Convenient 10 µl reaction set up
GoodLR Clonase™ Enzyme Enzyme
5X reaction buffer
  • Basic cloning of only single fragments
BestBP Clonase™ II Enzyme MixSingle mix of enzyme and reaction buffer
  • High efficiency cloning for Entry Clone construction
  • Single mix format eliminates pippetting steps and error
  • Easy to use single mix format ensures enzyme stability
  • Convenient 10µl reaction set up
GoodBP Clonase™ EnzymeEnzyme
5X reaction buffer
  • Basic cloning of only single fragments