Gateway® Cloning Technology

The easy-to-use choice for everyday cloning

Gateway® recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. From protein expression to functional analysis, Gateway® cloning technology is applicable for a variety of research areas, for truly multidisciplinary scientific studies.

• Fast—1-hour room temperature cloning reactions
• Accurate—cloning efficiency >99% delivers the clone you need
• Easy—no need for restriction enzymes or ligation to maintain orientations and reading frames for expression-ready clones
• Convenient—no resequencing required; use the same clone from target identification to validation for consistency
• Flexible—shuttle DNA inserts from one expression vector to the next
• Versatile—select from E. coli, yeast, insect, or mammalian cell Destination vectors (vector conversion is also available)

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