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Cloning 2

Gateway® Cloning

Gateway Cloning Gateway® recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system. Widely adopted in the research community with more than 1,500 references since its launch, Gateway® technology makes collaboration across research disciplines easy and convenient and enables access to a multitude of vectors from these research groups for truly multidisciplinary scientific studies.
Finally, new advancements such as MultiSite Gateway® technology makes Invitrogen’s Gateway® cloning the ideal cloning method for protein expression and functional analysis.

  • Fast, one hour, room temperature cloning reactions with >99% efficiency deliver the clone you need
  • Maintain orientation and reading frame without using restriction enzymes or ligation makes expression-ready clones
  • Eliminate resequencing ensures consistent results throughout your experiment using the same clone from target identification to validation
  • Shuttle insert DNA from one expression vector to another affords flexibility while simplifying your cloning workflow

Learn more about Gateway® cloning technology

Entry Vectors & Kits

pENTR vectors are available with efficient Directional TOPO® cloning and classic restriction enzyme and ligase cloning.

Not sure which Gateway® Entry vector to use?


Gateway® Vector Conversion Systems

Making your vector Gateway® compatible simplifies your cloning process while enabling access to an extensive collection of vectors.


Gateway® Recombination Enzymes

BP and LR Clonase™ II enzymes use a single mix format to mediate a robust recombination cloning reaction to create Entry and Expression clones.


Donor Vectors

Your PCR insert with attB sites is the ideal substrate for efficient BP recombination cloning into  classic pDONR™ vectors, creating an Entry clone.

Destination Vectors

An unparalleled selection of Gateway® Destination vectors from expression of proteins in various cells to conducting RNAi studies, crystallography, and protein-protein interaction functional studies.


MultiSite Gateway®

MultiSite Gateway® technology enables easy, accurate and efficient assembly of multiple DNA fragments in the order and orientation into a single vector.