Invitrogen Life Science > Products & Services > Applications > Clinical & Diagnostic Applications > Bead-Based IVD Assays > Bead-Based Immunoassay IVD

Dynabeads® for Immunoassay IVD

Dynabeads® offer many unique benefits and are well established as the gold standard immunodiagnostic method. A comprehensive range of products for use in immunodiagnostics is available, across different sizes and surface chemistries. Some Dynabeads® are optimized for immobilization of peptides and small antigens for antigen-down assays. Other Dynabeads® allow immobilization of larger proteins and antibodies, for use as a solid-phase in traditional immunoassays. Some of the products are from our existing bio-discovery portfolio, while others are developed and supplied on an OEM basis.

Benefits include

- High capacity & wide dynamic range
- High signal-to-noise ratio
- Adaptable to any known detection method & assay methodology
- Efficient & reproducible ligand immobilization
- Easy assay development
- Direct or indirect capture approach
- No magnetic remanence
- Excellent kinetics & shorter assay times
- Reproducible automated behavior
- Fast and efficient washing
- Increased sensitivity
- Long reagent stability on the immunoassay analyzer
- Easy handling in manufacturing and shortened assay development time

Product	Characteristics	Selected Applications
Dynabeads® MyOne™ Tosylactivated	Hydrophobic bead surface Size: 1 micrometer, monodisperse Iron content (Ferrites): 26% (37%) Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads Active chemical functionality: 0.04-0.06 mmol/g beads Direct covalent binding of proteins/peptides via primary amino- or sulphydryl groups. No further surface activation required.	Immunoassays and immunodiagnostics Protein and peptide purification Phage display and biopanning Cell separation
Dynabeads® M-280 Tosylactivated	Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups Size: 2.8 micrometer, monodisperse Iron content (Ferrites): 26% (17%) Active chemical functionality: 0.1-0.2 mmol/g beads Direct covalent binding of proteins/peptides via primary amino- or sulphydryl groups. No further surface activation required.	Immunoassays and immunodiagnostics Protein and peptide purification Phage display and biopanning Cell separation
Dynabeads® MyOne™ Streptavidin T1	Hydrophobic bead surface Based on tosylactivated beads (T1) Size: 1 micrometer, monodisperse BSA as blocking protein Isoelectric point: pH 5.0 Low charge (-10 mV, at pH 7) Iron content (Ferrites): 26% (37%) Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads Free biotin binding: > 1,100-1,700 pmoles/mg beads Binding biotinylated Ig: 40-50 µg/mg beads	Immunoassays and immunodiagnostics Isolation of biotinylated molecules Protein and peptide purification Phage display and biopanning Cell separation
Dynabeads® M-280 Streptavidin	Hydrophobic bead surface Based on tosylactivated beads Size: 2.8 micrometer, monodisperse BSA as blocking protein Isoelectric point: pH 5.0 Low charge (-10 mV, at pH 7) Iron content (Ferrites): 12% (17%) Free biotin binding: 650-900 pmoles/mg beads Binding biotinylated Ig: 5-10 µg/mg beads	Immunoassays and immunodiagnostics Isolation of biotinylated molecules Protein and peptide purification Phage display and biopanning Cell isolation
Dynabeads® M-280 Sheep anti-Rabbit IgG	Hydrophobic bead surface Coated with affinity purified sheep polyclonal against all rabbit IgG subclasses. Size: 2.8 micrometer, monodisperse Iron content (Ferrites): 12% (17%) Mostly Fc reactive Binding capacity: 0.1-1 µg Ig/10⁷ beads	Immunoassays and immunodiagnostics Protein and peptide purification Phage display and biopanning Cell isolation
Dynabeads® M-270 Streptavidin	Hydrophilic bead surface Based on carboxylic acid beads Size: 2.8 micrometer, monodisperse No blocking proteins used Isoelectric point: pH 4.5 Highly charged (-50 mV, at pH 7) Iron content (Ferrites): 14% (20%) Low aggregation of beads in high salt solutions Free biotin binding: 650-1,350 pmoles/mg beads Binding biotinylated Ig: 5-10 µg/mg beads	Immunoassays and immunodiagnostics with hydrophobic targets Isolation of biotinylated molecules Sequence specific DNA/RNA capture in nucleic acid based diagnostics For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
Dynabeads® M-270 Carboxylic Acid	Hydrophilic bead surface with carboxylic acid groups Size: 2.8 micrometer, monodisperse Iron content (Ferrites): 26% (37%) Very low aggregation Active chemical functionality 150 µmol/g beads	Immunoassays and immunodiagnostics Sequence specific DNA/RNA capture in nucleic acid based diagnostics Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
Dynabeads® MyOne™ Streptavidin C1	Hydrophilic bead surface Based on carboxylic acid beads (C1) Size: 1 micrometer, monodisperse No blocking proteins used Tween 20 in the buffer Isoelectric point: pH 5.2 Medium charged (-35 mV, at pH 7) Iron content (Ferrites): 26% (37%) Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads Very low aggregation Free biotin binding: > 2,500 pmoles/mg beads Binding biotinylated Ig: 20-25 µg/mg beads	Immunoassays and immunodiagnostics Isolation of biotinylated molecules Sample preparation of proteins for mass spectrometry Sequence specific DNA/RNA capture in nucleic acid based diagnostics