Adiposomes
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| LipidTOX™ Deep Red neutral lipid stain | H34477 | 637/655 | ![]() | ![]() | ![]() | Developed for image-based high content screening (HCS) assays to characterize the toxic side effects of compounds on lipid metabolism in mammalian cell lines, and also for the monitoring of adipogenesis; highly specific for neutral lipids, supplied in ready-to-use formulations, require no wash steps | |
| LipidTOX™ Green neutral lipid stain | H34475 | 495/505 | ![]() | ![]() | ![]() | ||
| LipidTOX™ Red neutral lipid stain | H34476 | 577/609 | ![]() | ![]() | ![]() | ||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
Cytoplasm
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Calcein AM | C3100MP | 494/517 | ![]() | Best single probe available for use in assays for cell adhesion, chemotaxis, and multidrug resistance; has better cell retention in viable cells than fluorescein, carboxyfluorescein, and BCECF; tends to have brighter fluorescence in a number of mammalian cell types | |||
| CellTrace™ calcein red-orange AM | C34851 | 576/589 | ![]() | | Intrinsically fluorescent; a useful cell tracer and indicator of cell viability; can be used in combination with blueand green-fluorescent probes | ||
| CellTrace™ Far Red DDAO-SE | C34553 | 646/659 (pH-dependent spectra; values determined at pH 9) | ![]() | | A fixable, far red–fluorescent tracer for very long-term cell labeling; the succinimidyl ester (SE) reactive group forms a strong covalent attachment to primary amines that occur in proteins and other biomolecules on the inside and outside of cells | ||
| Organelle Lights™ Cyto-GFP | O36227 | 485/520 | ![]() | ![]() (paraformaldehyde) | Cytoplasmic-residing due to a C-terminal nuclear export sequence | ||
| CellTracker™ Blue CMAC | C2110 | 353/466 | ![]() | Many cell types loaded with Cell- Tracker™ probes remain fluorescent and viable 24 hours after loading, through cell division, and are not transferred to adjacent cells. An excellent choice for investigating cellular thiol levels, cell viability and cytotoxicity, transplantations, and cell fusion. Once inside the cell the CellTracker™ dye becomes impermeant and thus well retained with strong fluorescence and uniform cytoplasmic staining; simultaneous use of a CellTracker™ reagent and a calcium indicator may permit ratio measurements of intracellular calcium. | CellTracker™ Blue CMAC has a low pKa, which ensures that it will exhibit bright blue fluorescence in the cytoplasm at all physiological pH levels; viability in the presence of the reagent extends at least 72 hours | ||
| CellTracker™ Green CMFDA | C7025 | 492/517 | ![]() | CellTracker™ Green CMFDA has a relatively low pKa, which ensures that it will exhibit bright green fluorescence in the cytoplasm at all physiological pH levels; CellTracker™ Green CMFDA is nonfluorescent until esterase cleavage occurs | |||
| CellTracker™ Orange CMTMR | C2927 | 541/565 | ![]() | CellTracker™ Orange CMTMR has a low pKa, which ensures that it will exhibit bright orange fluorescence in the cytoplasm at all physiological pH levels; viability in the presence of the reagent extends at least 72 hours | |||
| CellTracker™ Red CMTPX | C34552 | 577/602 | ![]() | The longer-wavelength CellTracker™ Red CMTPX exhibits bright red fluorescence that is easily distinguished from that of blue-, green-, and far-red– fluorescent probes (including fluorescein-based CellTracker™ Green CMFDA); viability in the presence of the reagent extends at least 72 hours | |||
| Qtracker® 525 Cell Labeling Kit | Q25041 | 525 †† | ![]() | ![]() | Best used with rapidly dividing cells or cells that are endocytically active, including common cell lines such as HeLa, CHO, U188, and 3T3 | ||
| Qtracker® 565 Cell Labeling Kit | Q25031 | 565 †† | ![]() | ![]() | |||
| Qtracker® 585 Cell Labeling Kit | Q25001 | 585 †† | ![]() | ![]() | |||
| Qtracker® 605 Cell Labeling Kit | Q25011 | 605 †† | ![]() | ![]() | |||
| Qtracker® 655 Cell Labeling Kit | Q25021 | 655 †† | ![]() | ![]() | |||
| Qtracker® 705 Cell Labeling Kit | Q25061 | 705 †† | ![]() | ![]() | |||
| Qtracker® 800 Cell Labeling Kit | Q25071 | 800 †† | ![]() | ![]() | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
Cytoskeleton
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Alexa Fluor® 350 phalloidin | A22281 | 346/442 | ![]() | ![]() | Alexa Fluor® dye–labeled phalloidins bind to F-actin in nanomolar concentrations and show equal affinity for large and small filaments; live-cell staining requires liposome delivery or microinjection of conjugates, compromised membranes, or unlabeled phalloidins; contrast between stained and unstained areas is extremely large; phalloidin-labeled actin remains functional | ||
| Alexa Fluor® 488 phalloidin | A12379 | 495/519 | ![]() | ![]() | |||
| Alexa Fluor® 555 phalloidin | A34055 | 555/565 | ![]() | ![]() | |||
| Alexa Fluor® 594 phalloidin | A12381 | 581/609 | ![]() | ![]() | |||
| Alexa Fluor® 635 phalloidin | A34054 | 633/647 | ![]() | ![]() | |||
| Alexa Fluor® 647 phalloidin | A22287 | 650/668 | ![]() | ![]() | |||
| Alexa Fluor® 680 phalloidin | A22286 | 679/702 | ![]() | ![]() | |||
| Cellular Lights™ Actin-GFP | C10126 | 488/510 | ![]() | ![]() | Fusions of fluorescent proteins to the N-terminus of β-actin in mammalian cells; allow visualization of cytoskeletal components in live cells or cytoskeletal rearrangements in HCS cytotoxicity assays | ||
| Cellular Lights™ Actin-RFP | C10127 | 555/584 | ![]() | ![]() | |||
| Cellular Lights™ Tubulin-GFP | C10106 | 488/510 | ![]() | ![]() | Fusions of fluorescent proteins to the N-terminus of β-tubulin that allow live-cell imaging of microtubules; to monitor tubulin changes as an indicator of cytotoxicity; also for following cell division in transiently transduced cells, including primary and stem cells | ||
| Cellular Lights™ Tubulin-RFP | C10112 | 555/584 | ![]() | ![]() | |||
| Cellular Lights™ MAP4-GFP | C10105 | 488/510 | ![]() | ![]() | Fusions of fluorescent proteins to microtubule associated protein 4 (MAP4), enabling visualization of microtubules in live cells that can also readily be fixed for end point studies such as HCS-based cytotoxicity analyses | ||
| Cellular Lights™ MAP4-RFP | C10140 | 555/584 | ![]() | ![]() | |||
| Rhodamine phalloidin | R415 | 540/565 | ![]() | ![]() | Conjugated to a popular orange-fluorescent dye, tetramethylrhodamine (TRITC); convenient probe for labeling, identifying, and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments | ||
| TubulinTracker™ Green | T34075 | 494/522 | ![]() | Green-fluorescent staining of polymerized tubulin in live cells | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Cytoskeleton Antibodies & Secondary Detection Reagents
Endoplasmic reticulum
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Concanavalin A, Alexa Fluor® 594 conjugate | C11253 | 590/617 | ![]() | Brighter fluorescence than our Texas Red® dye conjugate of concanavalin A, and more photostable; concanavalin A selectively binds to α-mannopyranosyl and α-glucopyranosyl residues | |||
| ER-Tracker™ Green | E34251 | 504/511 | ![]() | ![]() | ER Tracker™ Green and Red are live-cell permeant and highly ER-selective BODIPY®-based dyes; when cells are stained using the provided protocol, staining patterns are partially retained after aldehyde fixation | ||
| ER-Tracker™ Red | E34250 | 587/615 | ![]() | ![]() | |||
| Organelle Lights™ ER-GFP | O36212 | 485/520 | ![]() | (paraformaldehyde) | Targeted specifically to nucleus via the ER signal sequence of calreticulin and KDEL (ER retention signal) | ||
| Organelle Lights™ ER-OFP | O36223 | 550/580 | ![]() | ![]() (paraformaldehyde) | |||
| SelectFX® Alexa Fluor® 488 ER Labeling Kit | S34200 | 495/519 | ![]() | Kit for ER labeling in fixed cells, providing a primary antibody directed against ER-associated disulfide isomerase (PDI), an Alexa Fluor® 488 dye-labeled secondary antibody, PBS, blocking solution, fixative and permeabilization solutions | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Endoplasmic reticulum Antibodies & Secondary Detection Reagents
Golgi complex
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | ||
|---|---|---|---|---|---|---|---|---|
| Anti–human golgin-97 antibody (detection with an Alexa Fluor® dye–labeled secondary antibody) | A21270 | NA | ![]() | Detects the peripheral membrane protein golgin-97 on cytoplasmic face of the Golgi complex; cross-reacts with the golgin-97 protein from most species | ||||
| BODIPY® FL C5-ceramide complexed to BSA | D3521, B22650 | 505/511 | ![]() | ![]() | Selective staining of the Golgi complex with applications for lipid metabolism and trafficking studies; brighter, more photostable than NBD conjugates; concentration-dependent shift from green to red in the Golgi complex; good for outlining cell boundaries for observation of morphogenetic movements and for measuring rates of lipid synthesis by Schwann cells | |||
| BODIPY® TR ceramide complexed to BSA | D7540, B34400 | 589/617 | ![]() | ![]() | Selective staining of the Golgi complex with applications for lipid trafficking studies, brighter and more photostable than the NBD conjugates; displays no concentration-dependent emission | |||
| Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 488 conjugate | L11271 | 495/519 | Lectins selectively bind to terminal α-N-acetylgalactosaminyl residues—an intermediate sugar added in O-linkage to serine and threonine residues in cis-Golgi cisternae and then substituted with galactose and sialic acid in the trans-Golgi; for each cell type or tissue it is necessary to empirically determine appropriate lectins and staining conditions | |||||
| Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 647 conjugate | L32454 | 650/668 | ||||||
| NBD C6-ceramide | N1154 | 466/536 | Classic structural marker for the Golgi complex | |||||
| Organelle Lights™ Golgi-GFP | O36215 | 485/520 | ![]() | ![]() (paraformaldehyde) | Fusion proteins targeted specifically to the Golgi complex via the human Golgi-resident enzyme N-acetylgalactosaminyltransferase- 2 | |||
| Organelle Lights™ Golgi-OFP | O36224 | 550/580 | ![]() | ![]() (paraformaldehyde) | ||||
| Organelle Lights™ Golgi-RFP | O10098 | 555/584 | ![]() | ![]() (paraformaldehyde) | ||||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | ||||||||
- View Golgi complex Antibodies & Secondary Detection Reagents
Intracellular membranes
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| CellTrace™ BODIPY® TR | C34556 | 598/625 | ![]() | ![]() | ![]() | Accumulates in cellular membranes including plasma membrane and organelles such as mitochondria, endoplasmic reticulum, and the Golgi apparatus; an excellent counterstain for cells and tissues that are expressing GFP | |
| Image-IT® LIVE Intracellular Membrane and Nuclear Labeling Kit | I34407 | 350/461 (Hoechst 33342), 598/625 (BODIPY® dye) | ![]() | ![]() | ![]() | Contains CellTrace™ BODIPY® TR methyl ester and Hoechst 33343 for selective staining of the intracellular membranes and nuclei of live GFP-transfected cells | |
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
Lysosomes
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | ||
|---|---|---|---|---|---|---|---|---|
| Image-iT® LIVE Lysosomal and Nuclear Labeling Kit | I34202 | 350/461 (Hoechst 33342), 577/590 (LysoTracker® Red DND-99) | ![]() | ![]() | Contains LysoTracker® Red DND-99 dye and Hoechst 33342 for highly selective staining of lysosomes and nucleus in live GFP-transfected cells | |||
| LysoTracker® Blue DND-22 | L7525 | 373/422 | ![]() | Highly selective for acidic organelles at nanomolar concentrations, shows minimal staining of mitochondria, for labeling and tracking of acidic organelles in live cells, loads rapidly | ||||
| LysoTracker® Green DND-26 | L7526 | 504/511 | ![]() | Is taken up very rapidly by live cells; imaging using this dye is best between 1 and 5 minutes after dye is added | ||||
| LysoTracker® Red DND-99 | L7528 | 577/590 | ![]() | ![]() | Rapid loading, highly selective for acidic organelles at nanomolar concentrations, show minimal staining of mitochondria, for labeling and tracking of acidic organelles in live cells | |||
| LysoSensor™ Blue DND-167 | L7533 | 373/425 | ![]() | LysoSensor™ dyes exhibit a pHdependent increase in fluorescence upon acidification (more specific than traditional organic stains); they come in multiple colors with different pKas and are used singly (or potentially in combination) to investigate the acidification of lysosomes and alterations of lysosomal function or trafficking that occur in cells | Nonfluorescent at pH 7; increased fluorescence in acidic range | |||
| LysoSensor™ Green DND-153 | L7534 | 442/505 | ![]() | Nonfluorescent at pH 7; increased fluorescence in acidic range | ||||
| LysoSensor™ Yellow/Blue dextran, 10,000 MW | L22460 | 335, 381/452, 521 | ![]() | Fluorescence changes from blue (near neutral pH) to yellow (in acidic compartments); not cell permeant (must be microinjected or loaded by endocytosis); compatible with microscopy and flow cytometry | ||||
| LysoSensor™ Yellow/Blue DND-160 | L7545 | 329, 384/440, 540 | ![]() | pH-dependent dual excitation and dual emission peaks for ratiometric imaging; yellow fluorescence in acidic organelles, blue fluorescence in less acidic organelles | ||||
| Organelle Lights™ Lysosomes-RFP | O10100 | 555/584 | ![]() | ![]() | Fusion of monomeric TagRFP to lysosomal associated membrane protein 1; for live-cell visualization of lysosomes and any process in which they are involved—phagocytosis, endocytosis, autophagy | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | ||||||||
Membrane trafficking
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Cellular Lights™ CSF1r-GFP | C10078 | 488/510 | ![]() | ![]() | BacMam fusion of GFP to the receptor for colony stimulating factor 1 for analysis of its internalization kinetics | ||
| Cellular Lights™ Exoc1-YFP | C10079 | 514/528 | ![]() | ![]() | Fusion of Venus to Exoc1, a component of the exocyst complex involved in targeting these vesicles to the plasma membrane; allows following movement of exocytic vesicles in live cells | ||
| Organelle Lights™ Endosomes-GFP | O10104 | 488/510 | ![]() | ![]() | Fusion of photostable Emerald GFP to Rab5a, an early endosomal marker; allows following live-cell endosomal movement; cells can also be fixed for end-point studies | ||
| Organelle Lights™ Synaptophysin-GFP | C10080 | 488/510 | ![]() | ![]() | Fusions of fluorescent proteins to synaptophysin; for live-cell studies of endocytosis, exocytosis, synaptic vesicles, and membrane–synaptic vesicle complexes | ||
| Organelle Lights™ Synaptophysin-RFP | O10138 | 555/584 | ![]() | ![]() | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
Mitochondria
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Anti-OxPhos Complex V inhibitor protein antibody (detection with an Alexa Fluor® dye–labeled secondary antibody) | A21355 | ![]() | One of the many Important antibody tools for investigating mitochondrial biogenesis and mitochondrion-related diseases by western blot, native gel electrophoresis, immunohistochemistry, and other techniques; detection by anti-mouse labeled antibodies | ||||
| Image-iT® LIVE Mitochondrial and Nuclear Labeling Kit | I34154 | 350/461 (Hoechst 33342), 579/599 (MitoTracker® Red) | ![]() | ![]() | Contains MitoTracker® Red CMXRos dye and Hoechst 33342 for highly selective mitochondrial and nuclear staining in live GFP-transfected cells | ||
| JC-1 | T3168 | 514/529 (monomer form), 485–589/590 (J-aggregate form) | ![]() | | Widely used cationic dye that is dependent upon mitochondrial membrane potential for accumulation; JC-1 monomer (low concentration) is green, whereas accumulation at higher concentration shifts emission to the red, allowing ratiometric membrane potential measurements | ||
| MitoTracker® Green FM | M7514 | 490/516 | ![]() | Staining does not appear driven by mitochondrial function. Useful counterstain for mitochondrial mass. Has been reported as showing specific staining after fixation in certain cells. | |||
| MitoTracker® Red FM | M22425 | 581/644 | ![]() | Good live-cell stains, and well suited for multicolor labeling experiments; all are well retained after fixation except MitoTracker® Red FM | |||
| MitoTracker® Orange CMTMRos | M7510 | 554/576 | ![]() | ![]() | |||
| MitoTracker® Red CMXRos | M7512 | 579/599 | ![]() | ![]() | |||
| MitoTracker® Deep Red FM | M22426 | 644/665 | ![]() | ![]() | |||
| MitoTracker® Orange CM-H2TMRos | M7511 | 554/576 | ![]() | ![]() | Does not fluoresce until it enters live cells where it is oxidized to the fluorescent mitochondrion-selective probe MitoTracker® Red CM-H and then sequestered in mitochondria | ||
| MitoTracker® Red CM-H2XRos | M7513 | 579/599 | ![]() | ![]() | |||
| Organelle Lights™ Mito-GFP | O36210 | 485/520 | ![]() | (paraformaldehyde) | Targeted specifically to mitochondria via the leader sequence of E1 pyruvate dehydrogenase | ||
| Organelle Lights™ Mito-OFP | O36222 | 550/580 | ![]() | (paraformaldehyde) | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Mitochondria Antibodies & Secondary Detection Reagents
Nuclear envelope
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Organelle Lights™ NE-GFP | O36213 | 485/520 | ![]() | ![]() (paraformaldehyde) | Targeted specifically to nucleus via the nesprin-1α C-terminal transmembrane domain | ||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Nuclear envelope, Nucleoli and Nucleus Antibodies & Secondary Detection Reagents
Nucleoli
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| SYTO® 81 Orange nucleic acid stain | S11362 | 530/544 | ![]() | ![]() | Nucleolar | ||
| SYTO® RNASelect™ Green | S32703 | 490/530 (bound to RNA) | ![]() | ![]() | RNA selective, exhibiting increased fluorescence with RNA binding, prominent nucleolar staining, weak mitochondrial staining | ||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Nuclear envelope, Nucleoli and Nucleus Antibodies & Secondary Detection Reagents
Nucleus
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| 7-AAD | A1310 | 546/647 | ![]() | Nuclear stain, GC selective, weakly permeant in some cell types | |||
| Cellular Lights™ Histone 2B-GFP | C10128 | 488/510 | ![]() | ![]() | Alternatives to DNA staining for localizing the nucleus and chromosomes; allow localization of histones and fol lowing of cell division in live cells | ||
| folCellular Lights™ Histone 2B-RFP | C10129 | 555/584 | ![]() | ![]() | |||
| DAPI | D1306 | 358/461 | ![]() | Excellent nuclear counterstain, useful for mycoplasma detection, semipermeant at high concentration | |||
| Ethidium homodimer 1 | E1169 | 528/617 | ![]() | One of the highest-affinity fluorescent probes available for nucleic acid staining; binds to dsDNA, ssDNA, RNA, and oligonucleotides with a significant fluorescence enhancement (>40-fold); the dye’s intercalation is not sequence selective | |||
| Hoechst 33342 | H1399, H3570 | 350/461 | ![]() | ![]() | Nuclear counterstain, water soluble, relatively nontoxic to cells, slightly more permeant than Hoechst 33258 | ||
| Organelle Lights™ Nuc-CFP | O36218 | 435/485 | ![]() | ![]() (paraformaldehyde) | Fluorescent proteins targeted specifically to nucleus via the SV40 nuclear localization sequence; allows visualization of the nucleus without utilizing DNA-binding dyes; can also be used as a segmentation marker in end-point HCS studies | ||
| Organelle Lights™ Nuc-GFP | O36209 | 485/520 | ![]() | ![]() (paraformaldehyde) | |||
| Organelle Lights™ Nuc-OFP | O36220 | 550/580 | ![]() | ![]() (paraformaldehyde) | |||
| Organelle Lights™ Nuc-YFP | O36219 | 500/535 | ![]() | ![]() (paraformaldehyde) | |||
| Organelle Lights™ Nuc-RFP | O10099 | 555/584 | ![]() | ![]() (paraformaldehyde) | |||
| Propidium iodide | P3566 | 530/625 | ![]() | One of the most commonly used fixed-cell nuclear stains | |||
| SelectFX® Nuclear Labeling Kit, for fixed cells (DAPI, SYTOX® Green, 7-AAD, TO-PRO®-3 iodide) | S33025 | Several | ![]() | A sampler kit of our most popular nuclear stains with optimized protocols for product use | |||
| SYTO® 9 green fluorescent nucleic acid stain | S34854 | 483/503 | ![]() | ![]() | ![]() | Live-cell SYTO® dyes are permeant to eukaryotic and prokaryotic membranes, exhibit affinity for DNA and RNA, and display enhanced fluorescence upon binding | Live-cell nuclear counterstain, useful for bacterial viability |
| SYTO® 14 green fluorescent nucleic acid stain | S7576 | 517/549 (DNA), 521/547 (RNA) | ![]() | ![]() | ![]() | Live-cell nuclear counterstain, twice as fluorescent when complexed with RNA as with DNA | |
| SYTO® 82 orange fluorescent nucleic acid stain | S11363 | 541/560 | ![]() | ![]() | ![]() | Live-cell nuclear counterstain | |
| SYTO® 59 red fluorescent nucleic acid stain | S11341 | 622/645 | ![]() | ![]() | ![]() | Live-cell nuclear counterstain | |
| SYTO® 61 red fluorescent nucleic acid stain | S11343 | 628/645 | ![]() | ![]() | ![]() | Live-cell nuclear counterstain, live and dead bacterial stain | |
| SYTOX® Green nucleic acid stain | S7020 | 504/523 | ![]() | Highest-affinity SYTOX® stain, fixed-cell stain, useful as a dead-cell stain, 500× increase in fluorescence upon nucleic acid binding, can be used in conjunction with blue and red stains for multiplexing | |||
| SYTOX® Orange nucleic acid stain | S11368 | 547/570 | ![]() | Fixed-cell nuclear stain, some mitochondrial staining, shorter-wavelength emission than propidium iodide, useful as a dead-cell stain, 500× increase in fluorescence upon nucleic acid binding, can be used in conjunction with blue and green stains for multiplexing | |||
| TO-PRO®-3 iodide | T3605 | 642/661 | ![]() | Ultrasensitive dsDNA detection, dead-cell stain, electrophoresis prestain, used as an alternative to annexin conjugates for analyzing apoptotic cells | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Nuclear envelope, Nucleoli and Nucleus Antibodies & Secondary Detection Reagents
Peroxisomes
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes |
|---|---|---|---|---|---|---|
| Organelle Lights™ Peroxi-GFP | O36211 | 485/520 | ![]() | ![]() (paraformaldehyde) | Targeted specifically to peroxisome, via the peroxisomal C-terminal targeting sequence | |
| Organelle Lights™ Peroxi-OFP | O36225 | 550/580 | ![]() | ![]() | ||
| SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit | S34201 | 495/519 | ![]() | Kit for peroxisome labeling in fixed cells, providing a primary antibody directed against peroxisomal membrane protein 70 (PMP 70), an Alexa Fluor® 488–labeled secondary antibody, PBS, blocking solution, fixative, and permeabilization solutions | ||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | ||||||
- View Peroxisomes Antibodies & Secondary Detection Reagents
Plasma membrane
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| Alexa Fluor® 350 WGA | W11263 | 346/442 | ![]() | ![]() | ![]() | Alexa Fluor® labeled wheat germ agglutinins are versatile cationic probes for detecting glycoconjugates, i.e., N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues on cell membranes; useful in fixed and live cells that have not been permeabilized; best if fixed rapidly after staining | |
| Alexa Fluor® 488 WGA | W11261 | 495/519 | ![]() | ![]() | ![]() | ||
| Alexa Fluor® 555 WGA | W32464 | 555/565 | ![]() | ![]() | ![]() | ||
| Alexa Fluor® 594 WGA | W11262 | 590/617 | ![]() | ![]() | ![]() | ||
| Alexa Fluor® 633 WGA | W21404 | 632/647 | ![]() | ![]() | ![]() | ||
| Alexa Fluor® 647 WGA | W32466 | 650/665 | ![]() | ![]() | ![]() | ||
| Alexa Fluor® 680 WGA | W32465 | 679/702 | ![]() | ![]() | ![]() | ||
| CellTracker™ CM-DiI | C7001 | 553/570 | ![]() | ![]() | Bright, photostable, with excellent cellular retention; particularly suitable for cell tracking and long-term labeling of intracellular membranes, liposomes, viruses, and lipoproteins; aldehyde fixable | ||
| FM® 1-43FX | F35355 | 510/626 | ![]() | ![]() | FM®1-43 FX and FM®4-64FX exhibit substantially enhanced fluorescence upon insertion into the membrane and can be used for plasma membrane labeling when fixative is applied quickly after cell labeling. They are used to label the plasma membrane and to study vesiculation; they are easily applied to cells, nontoxic, water soluble, aldehyde fixable, and nonfluorescent in aqueous environments | ||
| FM® 4-64FX | F34653 | 565/744 | ![]() | ![]() | |||
| Image-iT® LIVE Plasma Membrane and Nuclear Labeling Kit | I34406 | 350/461 (Hoechst 33342), 590/617 (Alexa Fluor® 594 WGA) | ![]() | ![]() | ![]() | Contains Alexa Fluor® 594 WGA and Hoechst 33343 for selective staining of the plasma membrane and nucleus of live GFP-transfected cells | |
| Lipophilic Tracer Sampler Kit (DiI, DiO, DiR, DiD, DiA) | L7781 | Several | ![]() | ![]() | An assortment of the lipophilic carbocyanine dyes commonly used for membrane staining | ||
| Organelle Lights™ PM-CFP | O36216 | 435/485 | ![]() | ![]() (paraformaldehyde) | Fluorescent proteins targeted to the plasma membrane through the myristoylation/palmitoylation sequence from Lck tyrosine kinase; bright, specific plasma membrane staining | ||
| Organelle Lights™ PM-GFP | O36214 | 485/520 | ![]() | ![]() (paraformaldehyde) | |||
| Organelle Lights™ PM-OFP | O36226 | 550/580 | ![]() | ![]() (paraformaldehyde) | |||
| Organelle Lights™ PM-RFP | O10139 | 555/584 | ![]() | ![]() (paraformaldehyde) | |||
| Organelle Lights™ PM-YFP | O36217 | 500/535 | ![]() | ![]() (paraformaldehyde) | |||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||
- View Plasma membrane Antibodies & Secondary Detection Reagents
Whole-cell stains for image segmentation in HCS applications
| Product | Cat. no. Emission Color* | Ex/Em† | Live‡ | Fixed§ | Fixable** | Key attributes | |
|---|---|---|---|---|---|---|---|
| CellMask™ Blue stain | H34558 | 346/442 | ![]() | Label cytoplasm and nucleus via nucleic acid binding; no wash steps are required, and imaging can be performed in stain solution; validated as a counterstain and a segmentation tool for HCS applications; CellMask™ Red stain can be adjusted to give more prominent nuclear labeling or an overall cell stain, depending on the concentration used | |||
| CellMask™ Deep Red stain | H34560 | 650/665 | ![]() | ||||
| CellMask™ Red stain | H32711 | 622/645 | ![]() | ||||
| * Gray represents fluoresence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable. | |||||||




