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Cell and Tissue Analysis

Cell Isolation with Dynabeads® Technology

Gentle and tube-based magnetic separation with Dynabeads® is the technology of choice when you want to isolate high yields of pure, viable and functional cells. Your cells are not exposed to the stress of going through a dense column, and there's no risk of your results being affected by the isolation method.

You can easily isolate any cell subset using Dynabeads® and a DynaMag™ magnet. Your specific product of choice will depend on the species and the isolation strategy:

Selection Guides for Cell Isolation Products:




Human Cell Separation
  • Directly from whole blood, MNC, buffy coat, bone marrow or tissue samples.




Mouse Cell Separation
  • Directly from bone marrow, spleen, lymph node or thymus 




Separation of other cell types
  • Dynabeads® are used for cell isolations from of a range of different species and cell subsets.


Positive Isolation
  • Ideal for flow-analysis and cell-based assays
  • When maximum purity and yield is critical 
  • From whole blood, bone marrow or buffy coat



Negative Isolation
  • Truly untouched cells, bead- and antibody-free
  • In perfect shape for any application
  • High yield, purity and viability



Depletion
  • Easy removal of unwanted cell types
  • Ideal for positive cell isolation for molecular applications
  • High yield and purity

Product catalog pages for:

Human cell separation

Mouse cell separation

Secondary cell separation

Positive Cell Isolation

In positive isolation, Dynabeads® bind the target cells and the bead/cell complexes are pulled to the magnet (DynaMag™). The supernatant is discarded, leaving the pure target cells behind,
The beads can easily be removed using the innovative Dynabeads® FlowComp™ technology. A biotin/streptavidin based mechanism allows for fast, simple and stress-free release of cells after isolation. Your cells are no longer attached to iron particles after isolation.

Other Dynabeads® release systems include CELLection™ technology (employs a DNA linker), and DETACHaBEAD™ technology (gives bead- and antibody-free cells). 		  

Click to see Workflow

click image to see FlowComp™ workflow


Negative Cell Isolation

In negative isolation, Dynabeads® are used to deplete all the unwanted cells using the supplied antibody mix.  Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometers and used in any functional assay. Click here to learn more about negative cell separation .

Should you want to refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary-coated Dynabeads®  you can create your own negative isolation kit for isolation of any cells from any species.

Depletion

In depletion, cell-specific Dynabeads® are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant - still containing all your wanted cells - for downstream assays. Click for more info on: Dynabeads® for human cell depletion and mouse cell depletion.

You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.
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