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Cell Culture

Keratinocyte Culture Systems

Keratinocyte Culture Systems Primary Human Keratinocyte Cells adult (HEKa)
Primary Human Keratinocyte Cells neonatal (HEKn)
  • > 500,000 cells/vial cryopreserved at the end of the 1' culture
  • > 70% viability
  • > 25 population doublings HEKa (adult)
  • > 30 population doublings HEKn (neonatal)
  • Optimized for superior performance over the leading competitors

Looking for more information?  Checkout our Introduction to Cutaneous Biology!

For a comprehensive list of cells, technical resources and related technologies visit the Cell Resource Center.

Phase contrast images of Human Epidermal Keratinocytes (HEKa) grown in culture

HEKa cells grown in EpiLife (M-EPI-500-CA) with the addition of Human Keratinocyte Growth Supplement (HKGS). First culture after thaw.

Day 3 - HEKa

Day 5 - HEKa

Day 7 - HEKa

Get more life out of you cells

GIBCO EpiLife serum-free cell culture medium
  • Defined, animal origin-free medium
  • Extends the in vitro lifespan of primary cells up to two times compared to other serum-free media
  • Ease of use with single-shot supplementation




  • Figure 1    - Normal neonatal human keratinocytes (HEKn) (C-001-5C) were grown in EpiLife medium and in a keratinocyte medium from a leading competitor. Cultures grown in EpiLife medium demonstrated population doublings over an extended period compared to cells grown in the competitor's medium.

Keratinocyte Product Selection Guide

If you'd like to culture your cells....

  • in an animal-product free (APF) environment
  • for an extended lifespan




  • in a chemically-defined environment
  • for an extended lifespan



  • in a Bovine Pituitary Extract (BPE) containing environment
  • for an extended lifespan



  • in a Bovine Pituitary Extract (BPE) containing environment
  • for a standard lifespan
Then choose: